| 周敏明,俞昌喜,王妙珍,曹小定,吴根诚.褪黑素合用电针时大鼠脑内β-内啡肽的变化[J].中国中西医结合杂志,2001,(2):115-118 |
| 褪黑素合用电针时大鼠脑内β-内啡肽的变化 |
| Changes of Cerebral β-Endorphin in Rats Treated with Combination Therapy of Melatonin and Electroacupuncture |
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| DOI： |
| 中文关键词: 褪黑素 电针镇痛 前阿黑皮原 β-内啡肽 免疫组织化学 原位杂交 |
| 英文关键词:melatonin electroacupuncture analgesia proopimelanocortin β endorphin immuno histochemistry in situ hybridization |
| 基金项目:“九五”国家科技攻关资助课题!(No .96— 90 6— 1 1— 0 1 ) |
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| 中文摘要: |
| 目的 :观察褪黑素合用电针对大鼠下丘脑弓状核中 β 内啡肽样免疫阳性反应强度及其前体———前阿黑皮原 (POMC)mRNA表达的影响 ,以探讨褪黑素加强电针镇痛的机理。方法 :采用ABC免疫组织化学和原位杂交组织化学技术 ,结合计算机图像处理 ,测定其积分光密度 (IOD)值。结果 :腹腔注射褪黑素 60mg/kg ,30min时合用电针 30min后 ,脑内 β 内啡肽样免疫阳性物质积分IOD值明显降低 ;10h后脑内POMCmRNA阳性物质积分IOD值明显增高。结论 :褪黑素加强电针镇痛效应的机制可能与促进脑内β 内啡肽的释放和合成有关 |
| 英文摘要: |
| Objective: To explore the strengthening of acupuncture analgesic mechanism on the level of β endorphin and proopimelanocortin mRNA expression in the arcuate nucleus of hypothalamus in rats following electroacupuncture (EA) combined with melatonin (MEL). Methods: Integrated optical density (IOD) was measured by ABC immuno histochemical and in situ hybridization technique with computerized image processing. The rat′s brain was coronally sectioned after combination of EA and MEL. Results: IOD of β endorphin like immunopositive substance in rat′s brain was lowered significantly, which was measured after MEL (60 mg/kg) was injected intraperitoneally and followed by EA 30min later for 30min, and the IOD of cerebral POMC mRNA positive substance increased significantly 10 hrs later. Conclusion: The mechanism of MEL in enhancing EA analgesic effect might be related with the release and synthesis of β endorphin. |
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