粉防己碱对小鼠吗啡诱导的高活性和强化效应的影响

目的 :研究粉防己碱 (Tet)对小鼠吗啡诱导的高活性和强化效应的影响。方法 :单次给予吗啡用自主活动仪检测运动活性 ,利用位置偏爱实验评估吗啡的强化效应 ,用爬壁实验考察与多巴胺系统的关系 ,利用免疫组织化学方法显示脑内即刻早期基因c fos的表达情况。结果 :吗啡单次处理小鼠可诱导高活性 ,重复处理可产生位置偏爱 ,Tet 30mg/kg或 60mg/kg皮下注射可抑制吗啡诱导的高活性 ,60mg/kg抑制位置偏爱效应 ,而不影响小鼠的爬壁反应 ,Tet可抑制吗啡位置偏爱模型形成后伏隔核、腹侧被盖区及前额皮层c fos阳性细胞的表达。结论 :Tet能抑制吗啡诱导的高活性及强化效应 ,其效应可能与抑制特定脑区即刻早期基因c fos的表达有关

英文摘要:

Objective: After single administration of morphine and the motion activity was measured by ambulometer, conditioned place preference paradigm was used to study the reinforcing effect of morphine, climbing behavior was used to evaluate the relation with Dopaminergic system and immediate early expression of c fos gene was in brain was showed by immunohistochemical method. Results: Single administration of morphine could induce hyperactivity, repeated treatment would produce a conditioned place preference response, tetrandrine 30 or 60 mg/kg hypodermic injection could inhibit the morphine induced hyperactivity, 60 mg/kg could inhibit the conditioned place preference response but no influence on climbing behavior in mice was found. Tetrandrine could inhibit the c fos gene expression in nucleus accumbens, ventral tegmental and prefrontal cortex in place preference model formed by morphine. Conclusion: Tetrandrine could inhibit the hyperactivity and conditioned place preference response induced by morphine, it might relate to reducing the c fos gene expression in special area of brain in mice.

关闭