抗震止痉胶囊对帕金森病作用机制的实验研究

作者	单位
鲍远程	安徽中医学院第一附属医院神经内科
汪瀚	安徽中医学院第一附属医院神经内科
张波	安徽中医学院第一附属医院神经内科
陈怀珍	安徽中医学院第一附属医院神经内科
王晓阳	安徽中医学院第一附属医院神经内科
徐国存	安徽中医学院第一附属医院神经内科
王艳昕	安徽中医学院第一附属医院神经内科
杨兴涛	安徽中医学院第一附属医院神经内科

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中文摘要:

目的 :探讨抗震止痉胶囊对帕金森病 (PD)大鼠作用机理。方法 :采用 6 羟基多巴胺微量注射损毁左侧黑质致密区复制帕金森病大鼠模型 ,将模型大鼠随机分为 3组 ,分别用抗震止痉胶囊 (中药组 )、左旋多巴 (西药组 )和生理盐水 (模型组 )治疗 30天 ,并检测各组大鼠治疗前后旋转行为 ,处死后测定各组左侧纹状体、黑质相关指标。结果 :中药组大鼠旋转行为有一定改善作用 (P <0 0 5) ;与模型组比较 ,纹状体多巴胺 (DA)含量、DA/高香草酸 (HVA)比值均增高 (P <0 0 1) ,黑质谷胱甘肽过氧化物酶、超氧化物歧化酶、谷胱甘肽均显著升高 (P <0 0 1) ,丙二醛降低 (P <0 0 1)。西药组大鼠旋转行为有明显改善 (P <0 0 1)。结论 :抗震止痉胶囊能部分改善PD大鼠旋转行为 ,提高纹状体DA含量及DA/HVA比值 ,其机制可能在于减轻自由基损害 ,减少DA分解代谢。

英文摘要:

Objective: To explore the therapeutic mechanism of Kangzhen Zhijing Capsule (KZZJC) on rats with Parkinson′s disease (PD). Methods: PD rat model was established by microinjection of 6 hydroxydopamine solution into left substantia nigra zona compacta (SNZC). Model rats were randomly divided into 3 groups and treated with KZZJC, levodopa and normal saline respectively for 30 days. The rotational behavior of rats before and after treatment was observed. Then rats were sacrificed and relevant indexes of left SNZC and striatum were examined. Results: In rats treated with KZZJC, the rotational behavior was improved (P<0 05), the levels of dopamine (DA) content and DA/homovanillic acid (HVA) ratio in striatum, and glutathione peroxidase, superoxide dismutase and glutathione in SNZC increased, but malondialdehyde in SNZC decreased, as compared with those in rats treated with normal saline, the difference was significant (P<0 01 or P<0 05). Rotational behavior in rats treated by levodopa was improved significantly (P<0 01). Conclusion: KZZJC could partially improve the rotational behavior in PD model rats, increase DA content and DA/HVA ratio in striatum. The mechanism might be the reducing of free radical damage and DA catabolism.

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