葛根素对大鼠脑及脏器组织一氧化氮体系的影响及其作用机制的探讨

作者	单位
吴平	重庆医科大学附属第一医院老年科!重庆400016
曾繁荣	重庆医科大学附属第一医院老年科!重庆400016
马厚勋	重庆医科大学附属第一医院老年科!重庆400016

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中文摘要:

目的 :研究葛根素对大鼠脑及脏器组织一氧化氮 (NO)含量、一氧化氮合酶 (NOS)活性的影响。方法 :16只大鼠随机分为两组 ,每组 8只。葛根素组大鼠腹腔注射葛根素每天 80mg/kg ,对照组注射等体积丙二醇 ,两组均连续用药 30天后 ,取大鼠心、脑、肝、肾组织 ;采用硝酸还原酶法测定NO含量 ,血红蛋白氧化法测定组织NOS的活性 ,同时对两组大鼠肝、脑组织作电镜超微结构观察。结果 :葛根素组大鼠心、脑、肝、肾组织NO含量较对照组明显增高 (P <0 0 5或P <0 0 1) ;两组大鼠仅心、脑组织中检测到NOS活性 ,且葛根素组心、脑组织NOS活性高于对照组 (P <0 0 5) ;电镜观察两组大鼠肝、脑组织超微结构均未见异常。结论 :葛根素增加组织NOS活性及NO含量 ,可能是其发挥药理作用的机制之一。

英文摘要:

Objective: To evaluate the effect of Puerarin on nitric oxide (NO) content and nitric oxide synthase (NOS) activity in rats′ tissue. Methods: Sixteen adult Wistar rats were randomly divided into two groups, 8 in each group. The Pur erarin group was intraperitoneal administered Puerarin 80 mg/kg·d and the control group was given propylene glycol by intraperitoneal injection for 30 days. The NO content in rats′ heart, brain, liver and kidney was determined by nitratase method and NOS was detected by oxyhemoglobin oxidation method. Besides, the electromicroscopic examination on rats′ liver and brain was also conducted. Results: NO content in heart, brain, liver and kidney in the Puerarin group was significantly higher than that in the control group respectively (P<0 05 or P<0 01). NOS was detected only in heart and brain in both groups and it was also higher in the Puerarin group than that in the control group (P<0 05). No abnormal ultrastructure was detected by electromicroscope in liver and brain of both groups. Conclusion: Increasing NO content and NOS activity in tissues may be one of the mechanism for pharmaceutical action of Puerarin.

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