五子衍宗丸及其拆方对老年大鼠心脑线粒体DNA缺失、线粒体呼吸链酶复合体及ATP合成的影响

Effect of Wuzi Yanzong Pill and Its Disassembled Prescription on Mitochondrial DNA Deletion, Respiratory Chain Complexes and ATP Synthesis in Aged Rats

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DOI：

中文关键词: 五子衍宗丸枸杞子菟丝子衰老线粒体DNA 呼吸链酶复合体三磷酸腺苷

英文关键词:Wuzi Yanzong pill Fructus Lycii Semen Cuscutae aging mitochondrial DNA respiratory chain complexes adenosine triphosphate

基金项目:国家自然科学基金!资助项目 (No .3980 0 1 99)

作者	单位
王学美	北京大学第一医院中西医结合研究所!北京100034
富宏	北京大学第一医院中西医结合研究所!北京100034
刘庚信	北京大学第一医院中西医结合研究所!北京100034

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中文摘要:

目的 :研究五子衍宗丸及其拆方对线粒体DNA缺失、线粒体呼吸链酶复合体及三磷酸腺苷(ATP)合成的影响。方法 :用聚合酶链反应 (PCR)、酶动力学和生物发光技术进行检测。结果 :五子衍宗丸及其拆方的枸杞子、菟丝子可减少老年大鼠脑组织线粒体DNA缺失 (P <0 0 1) ,提高脑线粒体呼吸链复合酶Ⅰ、Ⅳ活力和ATP的合成 (P <0 0 5,P <0 0 1) ;枸杞子、菟丝子还可减少老年大鼠心线粒体DNA缺失 (P<0 0 5,P <0 0 1)。结论 :五子衍宗丸、枸杞子、菟丝子对老年大鼠线粒体DNA的氧化损伤有保护作用。

英文摘要:

Objective: To investigate the effect of Wuzi Yanzong Pill (WZYZP) and its disassembled prescription on mitochondrial DNA deletions, respiratory chain complexes and ATP synthesis in aged rats. Methods: Animal experiments with polymerase chain reaction (PCR), enzyme kinetics and bioluminescence technique were conducted. Results: WZYZP and its disassembled prescription of Fructus Lycii and Semen Cuscutae could reduce the mitochondrial DNA deletions and raise the activities of mitochondrial respiratory chain complexes Ⅰ, Ⅳ and adenosine triphosphate (ATP) synthesis in aged rats′ brain; Fructus Lycii and Semen Cuscutae could also reduce the mitochondrial DNA deletions in aged rats′ heart (P<0 05, P<0 01). Conclusion: WZYZP, Fructus Lycii and Semen Cuscutae have protective effect on oxidative damage of mitochondrial DNA in aged rats.

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