补肾活血汤对小鼠体外受精及其早期胚胎发育的影响

作者	单位
杨桂云	江苏省中西医结合医院
王佩娟	江苏省中西医结合医院
贾晓斌	江苏省中西医结合医院
顾奎兴	江苏省肿瘤防治研究所
曹步凯	江苏省农业科学院胚胎工程研究室
王晓丽	江苏省农业科学院胚胎工程研究室
纪孝萍	江苏省农业科学院胚胎工程研究室

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中文摘要:

目的 :探讨补肾活血汤对女性生殖活动的影响 ,揭示其治疗不孕的作用机理。方法 :以补肾活血汤的兔含药血清作为培养基添加剂 ,分别与未交配小鼠的精、卵及交配后雌鼠体内回收的 2细胞期胚胎共同体外培养 ,观察小鼠体外受精及早期胚胎体外发育的变化。结果 :含药血清加入培养能显著提高小鼠的体外受精率 (P <0 0 1) ,并可促进其后的各期胚胎发育 ,尤其对 4细胞及 8细胞期胚胎的发育有显著促进作用 (P <0 0 5) ;对小鼠体内 2细胞期胚胎的体外 4细胞发育率无明显影响 ,而对其 8细胞期胚、桑椹胚、囊胚、孵化胚的各期发育率均有明显促进作用 (P <0 0 5或P <0 0 1)。结论 :补肾活血汤具有提高卵子受精率和促进早期胚胎发育的作用

英文摘要:

To explore the effect of Bushen Huoxue decoction (BSHX) on female reproduction and elucidate its therapeutic mechanism to infertility. Methods: The BSHX medicated serum of rabbit, as a supplement, was co cultured with the sperm and ovum of non copulated mice, and the 2 cell embryos of copulated female mice separately, to observe the changes of in vitro fertilization rate (IVF) and early embryogenesis rate. Results: By co cultured with BSHX medicated serum, IVF rate was increased obviously (P<0 01), and the follow up early embryogenesis rate at various period was promoted, particularly that of the 4 cell and 8 cell embryos (P<0 05). No influence on developmental rate of in vitro 4 cell embryos obtained from the in vivo 2 cell embryos. But the development of the 8 cell embryos, morula, blastula and hatching were promoted significantly. Conclusion: BSHX could raise the fertilization rate and promote the early embryonic development.

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