老年气滞血瘀、气虚血瘀患者与CD62p、CD63及TSP关系的临床观察

Clinical Study on Relationship of CD62p, CD63 and TSP to the Qi-Stagnation Induced Blood-Stasis Syndrome and Qi-Deficiency Induced Blood-Stasis Syndrome in Aged Patients

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中文关键词: 血瘀证中医分型血小板活化分子

英文关键词:Blood-Stasis Syndrome TCM typing platelet activating factor

基金项目:山东省科委资助课题 (No .991 2 0 570 8)

作者	单位
孔令钧	山东大学齐鲁医院!济南250012
李鲁扬	山东大学齐鲁医院!济南250012
唐占府	山东大学齐鲁医院!济南250012

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中文摘要:

目的 :观察气滞血瘀、气虚血瘀与血小板活化的关系。方法 :采用流式细胞仪和特异性抗活化血小板单克隆抗体对气滞血瘀、气虚血瘀患者 118例的血小板活化分子α 颗粒膜蛋白 (CD6 2 p)、溶酶体完整膜蛋白 (CD6 3)、凝血酶敏感蛋白 (TSP)的表达量进行定量研究 ,同时测定血小板聚集功能。结果 :气滞血瘀、气虚血瘀患者的CD6 2p、CD6 3、TSP的表达量皆显著高于正常对照组 (30名 ,均P <0 0 1) ;气滞血瘀组CD6 2 p、CD6 3、TSP的表达量皆显著高于气虚血瘀组 (均P <0 0 1) ;CD6 2 p与CD6 3、TSP均呈正相关 (r =0 74 0 ,P <0 0 1;r =0 74 4 ,P <0 0 1) ,TSP与CD6 3呈正相关 (r =0 6 35,P <0 0 1) ,CD6 2 p与ADP诱导的血小板聚集亦呈正相关 (r =0 715,P <0 0 1)。结论 :血小板活化参与了血瘀证的发生与发展 ;血小板活化尤其与气滞血瘀关系密切。

英文摘要:

Objective: To observe the relationship of platelet activation to the Qi-stagnation induced blood-stasis (QSBS) or Qi-deficiency induced blood-stasis (QDBS) syndrome. Methods: Expressions of platelet activating molecules, including α-granule membrane glycoprotein (CD62p), lysosomal integral membrane protein (CD63) and thrombospondin (TSP), in patients with QSBS and QDBS were determined quantitatively with flow-cytometry and specific monoclonal antibody against activated platelet. And platelet aggregation was tested simultaneously. Results: CD62p, CD63 and TSP expressions in Blood-Stasis patients, both QSBS and QDBS, were higher than those in the normal control significantly (all P<0.01); all the three expressions were higher in QSBS group than those in QDBS group (all P<0.01), Positive correlation was shown between CD62p and CD63 (r=0.740,P<0.01), CD62p and TSP (r=0.744,P<0.01),TSP and CD63 (r=0.635,P<0.01),and between CD62p and ADP induced platelet aggregation (r=0.715,P<0.01). Conclusion: Platelet activation was involved in the pathogenesis and development of Blood-Stasis Syndrome, especially closely related with the QSBS Syndrome.

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