复方丹参注射液防治大鼠环孢菌素A慢性肾毒性的实验研究

作者	单位
乔保平	河南医科大学第一附属医院泌尿外科
唐孝达	河南医科大学第一附属医院泌尿外科
阮翘	河南医科大学第一附属医院泌尿外科
李道明	上海第一人民医院泌尿外科

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中文摘要:

目的 :从基因水平探讨复方丹参注射液防治环孢菌素A(CsA)慢性肾毒性的机理。方法 :大鼠灌服CsA及低盐饮食 2 8天 ,用逆转录聚合酶链式反应 (RT PCR)的方法观察肾内转化生长因子 β1(TGF β1)、肾素的mRNA表达及TGF β1、Ⅳ型胶原 (CollagenⅣ )免疫组化的改变 ,并观察复方丹参注射液对上述改变的影响。结果 :CsA能诱导大鼠肾内TGF β1、肾素mRNA的高表达 ;并伴有CollagenⅣ肾内沉积增加。复方丹参注射液能减轻上述改变。结论 :复方丹参注射液对CsA的慢性肾毒性的防治与其减低肾素、TGF β1mRNA的高表达及减轻肾内CollagenⅣ的沉积有关。

英文摘要:

Objective: To explore the mechanism of protective effect of Compound Salvia Injection (CSI) on experimental cyclosporin A induced nephrotoxicity. Methods: Rats were on low-salt diet and cyclosporin A (CsA) was administered once a day through gastrogavage at dosage of 30mg/kg·d for 28 days. Expression of the mRNA for intrarenal transforming growth factor-β 1 (TGF-β 1) and renin was measured by reverse transcription-polymerase chain reaction (RT-PCR). Intrarenal expression of TGF-β 1 and Collagen Ⅳ was determined by immunohistochemical assays. The effects of CSI on these changes were also evaluated. Results: Chronic CsA-induced nephropathy might be correlated to TGF-β 1 and renin mRNA up-regulation as well as matric proteins accumulation in interstitium. CSI could reduce these changes. Conclusion: Decreased CsA-related TGF-β 1 and renin up-regulation expression and accumulation of matrix proteins in the kidney might be related to the protective mechanism of CSI on CsA-induced chronic nephrotoxicity.

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