中药调控腹膜淋巴孔机制的研究

作者	单位
吴妤	浙江大学细胞生物学和组织工程学研究所淋巴学研究室!杭州310006
李继承	浙江大学细胞生物学和组织工程学研究所淋巴学研究室!杭州310006
毛连根	浙江大学细胞生物学和组织工程学研究所淋巴学研究室!杭州310006
董晓巧	浙江大学细胞生物学和组织工程学研究所淋巴学研究室!杭州310006

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中文摘要:

目的 :研究由白术、丹参、党参、泽泻、益母草组成的中药复方治疗腹水的作用 ,阐明中药调控腹膜淋巴孔 ,促进腹水转归的机制。方法 :中药萃取、扫描电镜观察、计算机图像处理和定量分析。结果 :正常小鼠腹腔注射一氧化氮 (NO)供体后 ,腹膜淋巴孔显著扩大 (P <0 0 5 ) ,开放数目明显增加 (P <0 0 1)。中药灌胃 ,腹膜淋巴孔面积和开放数目均显著增加 (P <0 0 1)。中药灌胃后 ,再给NO合酶抑制剂 (L -硝基精氨酸 ) ,发现中药对腹膜淋巴孔的调控作用被明显逆转 :淋巴孔的面积和开放数目都显著减少 (P <0 0 1)。结论 :中药可能是通过升高内源性NO浓度 ,使腹膜淋巴孔开放 ,促进淋巴孔对腹水的转归 ,达到消腹水作用。

英文摘要:

Objective: To study the therapeutic effect of Chinese herbal medicines (CHM) in treating ascites to elucidate its mechanism in regulating the lymphatic stomata and promoting the absorption of ascites from the peritoneal cavity. Methods: Using scanning electron microscope (SEM) and computerized image processing and quantitative analysis assays, the CHM extract consisting of Atractylodes macrocephala, Salvia miltiorrhiza, Codonopsis pilosula, Alismatis orientale and Leonurus heterophyllus were studied. Results: Intraperitoneal injection of nitric oxide (NO) supplier or CHM administration could cause the average area of lymphatic stomata obviously enlarged (P<0 05), and the open numbers significantly increased (P<0 01) in normal healthy mice. When L notroarginine, a NO synthetase suppressor, was injected after CHM administration, it was found that the regulating effect of CHM on lymphatic stomata was inverted obviously, i.e. the average area and the density of lymphatic stomata were markedly reduced (P<0 01). Conclusion: CHM might treat ascites through increasing the endogenous NO concentration to open the lymphatic stomata and in turn to conduct the peritoneal water through lymphatic path.

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