补肾益智方对由A_β片段神经毒性诱导NG108-15细胞老年性痴呆模型神经递质释放影响的研究

作者	单位
钟振国	广西中医学院第二附属医院神经科学研究所
刘茂才	广州中医药大学老年脑病研究所
赖世隆	广州中医药大学老年脑病研究所
高洁	广州中医药大学老年脑病研究所
程淑意	广州中医药大学老年脑病研究所

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中文摘要:

目的 :观察补肾益智方对由Aβ片段神经毒性诱导的NG10 8- 15细胞老年性痴呆 (AD)模型神经递质释放的影响。方法 :利用免疫印迹检查、免疫放射活性测定及电生理学测定方法 ,观察补肾益智方含药血清处理Aβ片段神经毒性诱导的NG10 8- 15细胞后的胆碱乙酰基转移酶 (ChAT)活性、突触素蛋白(synapsin)水平及功能性突触形成率。结果 :补肾益智方含药血清组ChAT活性和突触素蛋白水平高于正常对照血清组 ;含药血清能调节递质释放能力和提高功能性突触形成。结论 :补肾益智方具有减轻细胞对Aβ的神经毒性反应 ,表明该方能拮抗AD的病理发展 ,并通过提高神经递质释放能力发挥对AD的治疗作用

英文摘要:

To observe the effect of Bushen Yizhi Recipe (BS YZR) on neurotransmitter release in Aβ segment neurotoxin induced NG108-15 ce llular model of Alzheimer disease (AD). Methods: The levels of choline acetyltransferase (ChAT) activi ty, synapsin and functional synapse formation rate in the cellular model treated with BSYZR containing serum were determined by Western blot analysis, immunorad iometric assay and electrophysiologic technique. Results: BSYZR containing serum treatment could cause increase of ChAT activity and synapsin level in model cells, as compared with those in n ormal control model cells treated with non drug containing serum, it also could regulate the release capacity of transmitter and raise the functional synapse f ormation. Conclusion: BSYZR could reduce the reaction of cell to Aβ neu rotoxin, indicating that it could be antagonistic to the pathological developmen t of AD by means of raising the neurotransmitter release capacity.

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