灯盏细辛注射液对自发性高血压大鼠心室及血管重构的影响

作者	单位
周建中	重庆医科大学附属第一医院心血管内科重庆400016
雷寒	重庆医科大学附属第一医院心血管内科重庆400016
陈运贞	重庆医科大学附属第一医院心血管内科重庆400016
李法琦	重庆医科大学附属第一医院心血管内科重庆400016

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中文摘要:

目的 :观察灯盏细辛注射液对自发性高血压大鼠 (SHR)心室及血管重构逆转作用。方法 :将10月龄SHR 2 4只按性别、体重、初始收缩压配对分为灯盏细辛、福辛普利、依那普利和生理盐水 (对照 ) 4组 ,各给药组给药剂量为 10mg·kg- 1·d- 1腹腔内注射 ,连续 8周。测定血压、心室重量指数及心肌细胞蛋白激酶C(PKC)活性 ,偏振光镜、透射电镜观察心脏、血管结构改变。图像分析计算心肌间质胶原面积及含量。结果 :灯盏细辛、福辛普利、依那普利组左心室肥厚有不同程度的消退 ,心率、右心室心脏重量指数无明显变化 (P >0 0 5 ) ,福辛普利、依那普利组降压及减低左心室心脏重量指数比灯盏细辛组明显 ,但心肌细胞肥大、变性等镜下结构改善灯盏细辛组比福辛普利、依那普利组更明显。 3种药物均能明显改善心肌间质及血管重构 ,灯盏细辛组心肌间质胶原面积、含量及胶原容积分数较对照组明显减少 ,3个用药组间差异无显著性(P >0 0 5 )。各用药组均能显著降低SHR心肌细胞膜PKC活性 (P <0 0 1) ,且灯盏细辛组较福辛普利、依那普利组更明显 (P <0 0 5 )。结论 :灯盏细辛可逆转SHR心肌、间质及血管重构 ,改善心肌僵硬度 ,具有心脏保护作用

英文摘要:

To observe the ventricular and vascular remod eling reversal eff ect of Erigeron breviscapus injection (EBI), a protein kinase C inhibitor, in spontaneous hypertension rats (SHR). Methods: Twenty four SHR were divided into 4 groups, they wer e treated respectively with EBI, Fosinopril, Enalapril and normal saline 10 mg/k g per day by intraperitoneal injection for 8 weeks. Systolic blood pressure (SBP ), ventricular weight index (VWI) and protein kinase C (PKC) activity in myocard ial cells were determined, ultrastructural changes of heart and vessel were obse r ved by polaroscope and transmission electron microscope, and the area and conten t of myocardial interstitial collagen (MIC) were determined by image analyzer sy stem. Results: The left ventricular hypertrophy was regressed to cer tain degree after EBI, Fosinopril and Enalapril treatment, but no significant ch ange in heart rate and right VWI was found. Fosinopril and Enalapril were superi or to EBI in lowering SBP and left VWI, and EBI was more obvious in improving my ocardial ultrastructure such as hypertrophy and degeneration. All the 3 drugs co uld improve the MIC and vascular remodeling, the MIC area, content and collagen volume fraction in the EBI group were lowered after treatment, as compared with those in the control group, but comparison between the three groups showed no si gnificant difference. The 3 drugs could reduce the PKC activity in myocardial ce ll membrane, and EBI showed the effect more significant than that of the other t wo (P<0 05). Conclusion: EBI could reverse the myocardial, interstitial and vascular remodeling, improve the rigidness of cardiac muscle, thus, has protect ive effect on heart.

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