补肾法延缓免疫衰老的临床与实验研究

作者	单位
沈自尹	复旦大学医学院中西医结合研究所上海200040
郑振	复旦大学医学院中西医结合研究所上海200040
郭为民	复旦大学医学院中西医结合研究所上海200040
陈伟华	复旦大学医学院中西医结合研究所上海200040
应健	复旦大学医学院中西医结合研究所上海200040

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中文摘要:

目的 :观察补肾法延缓免疫衰老的临床疗效及相应的实验研究。方法 :老年人 2 2对 (44名 ) ,采用随机分组 ,双盲给药 (补肾方即补肾益寿胶囊和安慰剂 ) ;实验研究则比较两个补肾方 (右归饮和补肾益寿胶囊 )和活血方 (桃红四物汤 )对老年大鼠T细胞凋亡及Fas、FasL基因表达的调控作用。结果 :临床结果显示补肾组T细胞凋亡率比安慰剂组明显降低 (P <0 0 1) ,FasL基因表达亦明显降低 (P <0 0 5 )。动物实验显示两个补肾方组老年大鼠T细胞凋亡率均明显降低 (P <0 0 5 ) ,FasL基因表达亦明显降低 (P <0 0 1) ,而活血方组均无变化。结论 :补肾法对老年人与老年大鼠T细胞FasL基因的转录具有负调控作用 ,这是补肾法下调老年人与老年大鼠T细胞过度凋亡的分子机理之一。

英文摘要:

Objective: To observe the clinical curative effect of Kidney tonifying method on retardation of immunosenescence and corresponding experimental study. Methods: A randomized double blind placebo controlled trial was used (RCT) on 22 pairs of aged subjects to elucidate the effect of Kidney tonifying recipe on the peripheral T lymphocyte apoptosis and the Fas/FasL gene expression in them. In rats experimental study, the effects of two kinds of Chinese recipes (Kidney tonifying recipe and blood circulation promoting recipe) on the same parameters as in clinical study as well as on cell apoptosis and gene expression regulation in old rats were also observed. Results: Clinical study showed that after treatment, the percentage of T lymphocyte apoptosis and the FasL gene expression in the Kidney tonifying group of aged subjects were lower than those in the placebo group (P<0 01). Animal experiment showed the same result as shown in clinical study in Kidney tonifying recipe treated rats, but not shown in those treated with blood circulation promoting recipe statistically. Conclusion: Kidney tonifying principle has down regulating effect on the transcription of apoptotic gene in both aged persons and old rats, this is one of the molecular mechanisms of Kidney tonifying method in decreasing over apoptosis in aged subjects and old rats.

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