补阳还五汤及其有效部位组方对沙土鼠脑缺血再灌注后脑组织热休克蛋白70表达的影响

Effect of Buyang Huanwu Decoction and Its Active Regions Combination on Brain Heat Shock Protein 70 Expression in Gerbils after Cerebral Ischemia/Reperfusion

免费下载全文查看/发表评论下载PDF阅读器

DOI：

中文关键词: 补阳还五汤有效部位组方脑缺血再灌注沙土鼠热休克蛋白 70

英文关键词:Buyang Huanwu Decoction active regions brain ischemia/reperfusion gerbil heat shock protein 70

基金项目:国家自然科学基金资助项目 (No .39770 893)

作者	单位
邓常青	广州中医药大学第一附属医院糖尿病研究所
王敏	湖南中医学院
贺福元	湖南中医学院

摘要点击次数: 1397

全文下载次数: 2143

中文摘要:

目的 :比较补阳还五汤及其有效部位组方对脑缺血后热休克蛋白 70 (HSP70 )表达的影响。方法 :采用沙土鼠双侧颈总动脉夹闭脑缺血模型 ,分别腹腔注射补阳还五汤及其有效部位组方 ,于缺血15min再灌注 4 8h后 ,以Northernblot检测脑组织HSP70mRNA的表达 ,以Westernblot检测其蛋白表达。结果 :沙土鼠脑缺血再灌注后 ,脑组织HSP70mRNA和蛋白表达显著增加 ,补阳还五汤及其有效部位组方可使脑缺血再灌注后HSP70mRNA表达减少 ,补阳还五汤的抑制作用强于有效部位组方 ;但两者对脑缺血再灌注后HSP70蛋白表达的增加均无明显影响。结论 :补阳还五汤及其有效部位组方可能通过抗缺血作用而抑制了缺血后HSP70mRNA的表达。

英文摘要:

Objective: To compare the effects of Buyang Huanwu Decoction (BYHWD) and its active regions combination on brain heat shock protein 70 (HSP70) expression. Methods: Cerebral ischemia model gerbils produced by ligating bilateral common carotid artery were injected intraperitoneally by BYHWD and its active regions combination separately. HSP70 mRNA expression was determined by Northern blot test and protein expression determined by Western blot test 48 hrs after ischemia for 15 min and reperfusion. Results: The level of HSP70 mRNA and protein expression in ischemic/reperfusion brain tissue increased significantly. BYHWD and its active regions combination could decrease the HSP70 mRNA expression obviously, the inhibition effect of BYHWD was more significant than that of its active regions combination, but neither of them showed any effect on HSP70 protein expression. Conclusion: BYHWD and its active regions combination might down regulate HSP70 mRNA expression in ischemic brain tissue through its anti ischemic effect.

关闭