银杏叶提取物对窒息新生儿红细胞免疫功能与血清脂质过氧化物的影响

目的 :观察新生儿窒息时红细胞免疫功能和血清脂质过氧化物的变化及银杏叶提取物(FGE)对其的影响及意义。方法 :窒息新生儿 30例随机分为FGE治疗组 (简称治疗组 )和非FGE治疗组(简称对照组 )各 15例 ,并设正常对照组 (简称正常组 ) 10名。均于生后 2 4h内抽血检测红细胞C3b受体花环率 (E C3bRR)、红细胞免疫复合物花环率 (E ICR)和全血超氧化物歧化酶 (SOD)活性及血清脂质过氧化物(LPO)水平 ,随后所有患儿均给予常规治疗 ,如吸氧、纠正酸中毒及维持水、电解质平衡 ,有惊厥者给予止惊、脱水等治疗 ;治疗组加达纳康 (银杏叶制剂 ) 15mg/(kg·d) ,分 2次口服 ,共服 7～ 8天。然后各组再次抽血进行上述指标检测及进行新生儿行为神经测定 (NBNA)。结果 :治疗前两组患儿E C3bRR和全血SOD活性显著低于正常组 (P <0 0 5 ) ,E ICR和血浆LPO水平显著高于正常组 (P <0 0 5 ) ;治疗后治疗组E C3bRR和SOD活性显著高于对照组 (P <0 0 5 ) ,E ICR和血浆LPO水平显著低于对照组 (P <0 0 5 ) ;治疗组NBNA评分明显高于对照组。结论 :新生儿窒息时红细胞免疫功能下降与其体内抗氧化能力下降、脂质过氧化损伤有关 ;银杏叶提取物可抑制自由基的产生和清除自由基 ,对抗细胞膜的脂质过氧化损伤并使红细胞免疫功能上调 ,具有?

英文摘要:

To observe the changes of erythrocyte immunity and serum lipid peroxide in asphyxia neonate, and to study the effect of Folium Ginkgo extract (FGE) on them. Methods: Thirty asphyxia neonates were randomly divided into 2 groups, the treated group and the control group, 15 in each group. Erythrocyte C 3b receptor rosette rate (E C 3bRR), erythrocyte immune complex rosette rate (E ICR), blood superoxide dismutase (SOD) activity and serum lipid peroxide (LPO) level were determined at 24 hrs after birth. Conventional treatment was given to both groups and FGE (15 mg/kg·d) was given to the treated group additionally for 7～8 days, then the above mentioned parameters were re examined and neonatal behavioral neurological assessment (NBNA) was measured as well. Results: E C 3bRR and SOD lowered, E ICR and serum LPO increased in the asphyxia neonate significantly (P<0 05). After treatment, comparison between the two groups showed that E C 3bRR and SOD were higher, E ICR and serum LPO were lower in the treated group than those in the control group, and NBNA scoring was obviously higher in the former than that in the latter (all P<0 05). Conclusion: Decrease of erythrocyte immunity in asphyxia neonate is related to the declined anti oxidation ability and lipid peroxidase injury. FGE could suppress the free radical production, scavenge free radicals, antagonize the lipid peroxidation injury of cell membrane and up regulate erythrocyte immunity. It displays the effects of nerve tissue protection and hypoxia ischemic brain injury alleviation.

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