大蒜素对急性脑梗死患者外周血白细胞表面粘附分子表达及变形性的影响

作者	单位
张久亮	中日友好医院中医心肾科
孙瑞娟	中日友好医院临床医学研究所
史载祥	中日友好医院中医心肾科
黄力	中日友好医院中医心肾科
杜金行	中日友好医院中医心肾科
毛建生	中日友好医院中医心肾科
曹启富	中日友好医院中医心肾科

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中文摘要:

目的 :观察大蒜素对急性脑梗死患者外周血白细胞粘附分子 (CD11a)表达及白细胞滤过指数 (变形性 )的影响。方法 :取患者及健康人外周血 ,分离粒细胞于体外 37℃孵育 ,采用抗体荧光标记 ,测定CD11a表达率 ;并测定粒细胞通过核孔滤膜的时间 ,并计算滤过指数。结果 :CD11a表达率 (% )健康人组(34 6 4± 2 5 34)与空白对照组 (5 5 35± 30 5 4)比较 ,差异有显著性 (P <0 0 5 ) ;大蒜素组 (49 16± 31 6 8)较空白对照组明显下降 (P <0 0 5 )。粒细胞滤过指数健康人组、空白对照组、大蒜素组及尼莫地平组分别为0 87± 0 4 6、6 4 2± 6 4 0、3 4 7± 3 6 7及 5 0 3± 3 72 ,大蒜素组与空白对照组比较 ,差异有显著性 (P <0 0 5 )。结论 :大蒜素可有效地抑制急性脑梗死患者外周血粒细胞CD11a表达率及改善其变形性

英文摘要:

Objective: To observe the effect of garlicin on adhension molecules CD11a and deformability of peripheral neutrophil in patients with acute cerebral infarction (ACI). Methods: Neutrophils were separated from peripheral blood of healthy subjects and ACI patients, and incubated in 37℃ in vitro. The CD11a expression was detected by antibody fluorescence labeling method and the time of neutrophils passing millipore membrane were measured for calculation of the filter index. Results: CD11a expression rate in healthy subjects was 34.64±25 34%, while in patients was 55.35±30.54%, difference between them was significant (P<0 05). After garlicin treatment, it lowered to 49.16±31.68%, as compared with untreated group, P<0 05. The neutrophil filter index in healthy group, untreated group, garlicin treated group and Nimodipine treated group was 0.87±0.46, 6.42±6.40, 3.47±3.67 and 5.03±3.72 respectively, comparison between that in the garlicin treated group and in untreated group showed significant difference (P<0 05). Conclusion: Garlicin could effectively inhibit the CD11a expression in peripheral blood neutrophils and improve the deformability of the neutrophils in ACI patients.

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