补阳还五汤抗缺氧再给氧乳鼠心肌细胞凋亡的实验研究

作者	单位
佟丽	第一军医大学中医系广州510515
沈剑刚	第一军医大学中医系广州510515
邱幸生	第一军医大学中医系广州510515
曲宏达	第一军医大学中医系广州510515
陈育尧	第一军医大学中医系广州510515

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中文摘要:

目的:观察含补阳还五汤的大鼠血清对缺氧24h再给氧4h后新生乳鼠心肌细胞凋亡的抑制作用及对一氧化氮（NO）含量的影响。方法:采用Annexin V-PI双标记方法（以流式细胞仪和ELISA法）检测细胞凋亡,以紫外分光光度法测定心肌细胞乳酸脱氢酶（LDH）释放水平,采用改良Yu方法测定NO浓度,用Ohkawa法测定硫代巴比妥酸（TBARS）反应物质含量。结果:补阳还五汤药物血清能显著抑制心肌细胞的凋亡及LDH释放,降低培养细胞上清液中NO和TBARS水平,其效应呈剂量依赖关系。结论:补阳还五汤具有抗缺氧再给氧心肌细胞凋亡作用,其机制与清除氧自由基和NO特性有关。

英文摘要:

Objective: To observe the preventive effect of rats’ serum containing Buyang Huanwu Decoction (BYH-WD) on cultured cardiomyocyte apoptosis of neonatal rat induced by means of 24 hrs hypoxia and 4 hrs reoxy-genation, and to investigate its mechanism concerned with nitric oxide (NO) . Methods: Myocyte apoptosis was detected by flow cytometry and ELISA with Annexin V-PI double labeled method. The lactate dehydrogenase (LDH) releasing level was measured with ultraviolet spectrophotometer. The NO concentration was determined by modified Yu method and the concentration of thiobarbituric acid response substance (TBARS) was tested by Ohkawa method. Results: BYHWD contained rats’ serum could significantly prevent cardiomyocyte from apoptosis induced by hypoxia and reoxygenation. After hypoxia-reoxygenation, the NO, LDH and TEARS levels in the supernatant of cultured liquid treated with BYHWD were significantly lower than those in non-treated cultured liquid, the effect of BYHWD was dose-dependent. Conclusion: BYHWD can prevent cardiomyocytes from apoptosis induced by hypoxia and reoxygenation, its mechanism might be related with oxygen free radical and NO scavenging produced during the hypoxia-reoxygenation process.

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