电针对大鼠脊髓损伤后层粘连蛋白表达的影响

目的:探讨电针治疗大鼠脊髓损伤（SCI）对损伤神经系统神经再生微环境中层粘连蛋白（laminin,LN）表达的影响。方法:用改良ALLEN氏致伤法制成大鼠胸12处脊髓不完全损伤模型,然后进行电针治疗,同时设立正常组、造模组与激素组作为实验对照;应用蛋白免疫印迹法观察不同时间点（1、2、4周）LN的表达。结果:电针组的LN从第1周起开始表达,2、4周水平呈逐渐升高的趋势;而激素组的LN从第2周起才开始有明显的表达,2、4周水平表达上调;电针组及激素组的LN表达均较造模组早,且电针组3个不同时间点的表达水平高于其他两组。结论:电针治疗可促进LN表达和产生,对中枢神经系统内在的再生潜力有积极的引导作用。

英文摘要:

Objective: To observe the effects of electro-acupuncture (EA) on laminin (LN) expression related to the nerve fibers regeneration and development in the microenvironment after rats’spinal cord injury (SCI). Methods: The SCI model of rats was established by the modified Allen’s method to injure the 12th thoracic vertebra of animal. Experimental rats were divided into 4 groups, the sham group (no SCI and treatment), control group (with SCI and no treatment), hormone group (with SCI and dexamethasone) and EA group (with SCI and EA). LN expression was assessed by Western Blot assay at various time points, week 1, 2 and 4. Results: The LN expression in EA group increased significantly at week 1, and remained constantly at week 2-4 with a tendency of gradually increasing. The LN expression in the hormone group elevated with obvious expression at week 2, and remained constantly also, with its expression up-regulated at week 2 - 4. The LN expression presented in both the EA group and the hormone group earlier than that in the control group, and the level of LN expression of EA group was higher than that in other two groups. Conclusion: EA could promote the production and secretion of LN expression, so it may have active beneficial leading effects on potentially regeneration capacity of the central nervous system.

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