电刺激对大鼠脑梗塞康复中星形细胞与神经元的影响

作者	单位
方燕南	中山大学附属第一医院广州510080
黄海威	中山大学附属第一医院广州510080
陶玉倩	中山大学附属第一医院广州510080
李玲	中山大学附属第一医院广州510080
丁明晖	中山大学附属第一医院广州510080
李花	中山大学附属第一医院广州510080
曾进胜	中山大学附属第一医院广州510080

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中文摘要:

目的:探讨大鼠急性脑梗塞后的康复机制。方法:用易卒中型双肾双夹型肾血管性高血压大鼠复制大脑中动脉闭塞模型,随机分为电刺激治疗组（简称治疗组）和对照组,用走平衡木法评价大鼠康复功能。于电刺激治疗后第1、3、6、9周末在脑梗塞灶边缘区观察神经元与星形胶质细胞的超微结构,胶质酸性蛋白表达,神经丝蛋白和微管相关蛋白2表达,神经元凋亡及脑微血管舒张情况。结果:治疗组从3～9周末瘫痪肢体功能恢复较对照组明显,在脑梗塞边缘区上述表达及脑微血管舒张数量均较对照组高（P<0.05）。结论:电刺激治疗可以促进瘫痪肢体功能恢复,其机理可能是电刺激增加星形胶质细胞增殖,增强神经元活性,激发脑微血管舒张。

英文摘要:

Objective: To explore the mechanism of rehabilitation after middle cerebral artery occlusion (MCAO). Methods: MCAO model was reproduced with two-kidney, two clip renovascular hypertensive rats stroke-prone (RHRSP), which were divided into two groups, the treated group (treated with electric stimulus) and the control group (untreated model) randomly. The rehabilitation of rats was evaluated by balance beam walking test. The ultrastructural changes of neurons and astrocytes, expressions of glial fibrillary acidic protein (GFAP)-posi-tive cells, neurofilament (NF) protein, and cerebral capillary dilatation M-associated protein-2 (MAP2), as well as the neurons apoptosis and the number of dilatation of cerebral capillary in the margin of infarcted area were observed by the end of 1st, 3rd, 6th and 9th week after modeling. Results: The motor function of paralysed limbs recovered better in the treated group than that in the control group by the end of 3 - 9 th week after MCAO, the expression of GFAP-positive cells in astrocytes and NF, MAP2 in neurons as well as the number of cerebral capillary dilatation at the margin of infarcted area were higher than those in the control group ( P < 0.05). Conclusion: Electric stimulation treatment could improve the recovery of motor function of paralyzed limbs. It might be due to the effect of electric stimulus in increasing astrocytes proliferation, reinforcing activity of neurons and evoking the dilatation of cerebral capillary.

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