新风胶囊对佐剂性关节炎大鼠疗效及滑膜fas、fasL及bcl-2表达的影响

Therapeutic Effect of Xinfeng Capsule in Treating Adjuvant Arthritis in Rats and Its Effect on fas, fasL and bcl-2 Expression in Synovial Membrane

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DOI：

中文关键词: 新风胶囊佐剂性关节炎基因表达

英文关键词:Xinfeng Capsule adjuvant arthritis gene expression

基金项目:卫生部优秀青年科研基金项目 (No .992 6)

作者	单位
刘健	安徽中医学院第一附属医院
张皖东	安徽中医学院第一附属医院
陈光亮	安徽中医学院
胡闻	安徽省立医院
韩明向	安徽中医学院第一附属医院

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中文摘要:

目的 :观察新风胶囊对佐剂性关节炎大鼠疗效及对病变滑膜fas、fasL、bcl 2表达的影响。方法 :采用弗氏完全佐剂造模 ,设立正常组、模型组、甲氨喋呤 (MTX)组、雷公藤 (TPT)组和新风胶囊 (XFC)组 ;记录各组大鼠的关节炎指数及体重变化 ;检测fas、fasL、bcl 2在局部关节滑膜组织中的表达。结果 :与治疗前比较 ,XFC组、TPT组及MTX组治疗后大鼠的关节炎指数显著降低 (P <0 0 1,P <0 0 5 )。XFC组给药前后体重增加值与正常组比较 ,差异无显著性 (P >0 0 5 ) ;TPT组及MTX组给药前后体重增加值显著低于正常组和XFC组 (P <0 0 1)。与模型组比较 ,XFC组fasL表达显著增加 (P <0 0 5 )、bcl 2、fas表达均有所减少 ,但差异无显著性 (P >0 0 5 ) ;XFC组上述数据与MTX组、TPT组比较差异无显著性 (P >0 0 5 )。结论 :XFC与MTX、TPT一样能降低佐剂性关节炎模型大鼠的关节炎指数 ,XFC在增加模型大鼠的体重方面优于MTX和TPT ;XFC可能通过促进fasL的表达、抑制bcl 2的表达 ,促进增生滑膜细胞的凋亡 ,达到抑制滑膜增生的目的。

英文摘要:

Objective: To observe the therapeutic effect of Xinfeng Capsule (XFC) in treating adjuvant arthritis of rats and its effect on fas, fasL and bcl 2 expression in synovial membrane. Methods: Rats were randomly divided into the normal group, the model group, the methotrexate (MTX) group, the TPT group and the XFC group. Except for the rats in the normal group, animals were modelled to adjuvant arthritis with Freund′s complete adjuvant, and the latter three groups were treated with MTX, Tripterygium wilfordii polysaccharide (TPT) and XFC respectively. The arthritis index (AI), change of body weight (BW) of rats were recorded, and the expressions of fas, fasL and bcl 2 in rats′ synovial membrane were determined. Results: Compared with before treatment, the AI in the three treated groups was lowered significantly (P<0 01, P<0 05). The BW increment in the XFC group after treatment was insignificantly different to that in the normal group (P>0 05), while it was significantly lower in the other two treated groups than that in the normal group and the XFC group (P<0 01). Compared with the model group, fasL expression was increased in the XFC group significantly (P<0 05), but bcl 2 expression decreased, fas expression showed insignificant difference (P>0 05). Comparison of the three gene expressions between the three treated groups showed no significant difference (P>0 05). Conclusion: XFC could decrease AI in rats with adjuvant arthritis in the same way as MTX and TPT, it effected better in increasing BW than the latter two. The effect of XFC might be performed by its action in enhancing fasL expression, inhibiting bcl 2 expression and promoting apoptosis of proliferated synovial membranous cell so as to restrain hyperplasia of synovial membrane.

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