调心方对快速老化模型小鼠海马学习记忆有关基因表达的影响

目的 :研究调心方对快速老化模型小鼠 (senescenceacceleratedmouse ,SAM )海马学习记忆有关基因表达的影响。方法 :应用逆转录聚合酶链反应 (RT PCR)的方法 ,以 β actin为内参照 ,检测SAM海马盐皮质激素受体 (MR)、早老蛋白 1(PS 1)、早老蛋白 2 (PS 2 )、tau、APP、apoE及bcl 2等学习记忆有关基因表达水平的变化及调心方对其影响。实验中选用E2 0 2 0为对照药物。结果 :与同龄抗快速老化的R系(SAMR1)比较 ,5月龄快速老化亚系 (SAMP8)海马MR、tau、PS 2、APP基因表达水平显著升高 ,apoE基因表达水平降低 ,而PS 1和bcl 2基因表达水平无明显变化 ;12月龄SAMP8海马MR、tau基因表达水平升高 ,bcl 2基因表达水平降低 ,而PS 1、PS 2、apoE、APP基因表达水平无明显变化。连续口服给予调心方对5月龄SAMP8海马MR、tau、apoE基因的异常表达具有纠正作用 ,对 12月龄SAMP8海马MR、bcl 2基因的表达异常亦具有纠正作用。结论 :连续口服给予调心方对 5月龄及 12月龄SAMP8海马学习记忆有关基因的异常表达有纠正或调节作用。

英文摘要:

Objective: To study the effect of Tiaoxin Recipe (TXR) on learning and memory related gene expression in hippocampus of senescence accelerated mice (SAM). Methods: Changes of learning and memory related gene expression, including mineralocorticoid receptor (MR), presenile protein 1 and 2 (PS 1, PS 2), tau, APP, apoE and bcl 2 in hippocampus of SAM were determined by reverse transcription polymerase chain reaction (RT PCR). The effect of TXR were tested. E2020 was used as the drug for control. Results: Compared with those in the same aged mice, in the 5 month old SAM, levels of gene expression of MR, tau, PS 2 and APP were significantly higher, that of apo E lower, levels of gene expression PS 1 and bcl 2 were unobviously changed; while in the 12 month old SAM, gene expression of MR and tau were higher, bcl 2 was lower and PS 1, PS 2, apoE and APP were also unobviously changed. Continuously orally taken TXR could correct the abnormality of MR, tau and apoE gene expression in hippocampus of 5 month SAM and that of MR and bcl 2 in 12 month SAM. Conclusion: Continuously orally taken of TXR has the effect of regulating and correcting learning and memory related gene expression in hippocampus of 5 month and 12 month SAM.

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