| 赵钢,张斌,陈建杰,王灵台.抗纤复方对人α1（Ⅰ）前胶原基因启动子活性的影响[J].中国中西医结合杂志,2002,(9):694-697 |
| 抗纤复方对人α1（Ⅰ）前胶原基因启动子活性的影响 |
| Effect of Anti-fibrosis Compound on Activity of Human α1（Ⅰ） Precollagen Gene Promoter |
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| DOI： |
| 中文关键词: 抗纤复方 胶原 启动子 转移生长因子β1 |
| 英文关键词:Anti fibrosis Compound collagen promoter transforming growth factor β 1 |
| 基金项目:国家自然科学基金项目 (No .39670 90 6) |
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| 摘要点击次数: 1149 |
| 全文下载次数: 1302 |
| 中文摘要: |
| 目的 :探讨抗纤复方 (AFC)对人α1(Ⅰ )前胶原基因启动子活性的作用及其机制。方法 :以含人α1(Ⅰ )前胶原基因上流 2 3kb ,0 8kb ,4 76bp ,173bp启动子片段为研究靶序列 ,以氯霉素乙酰基转移酶(CAT)为报告基因 ,构建基因重组质粒 ,转染成纤维细胞 (NIH3T3) ,建立稳定的α1(Ⅰ )前胶原启动子转染细胞体系 ,用抗纤复方和 (或 )转移生长因子 (TGFβ1)处理NIH3T3细胞 ,测定CAT活性。结果 :(1)抗纤复方能够抑制α1(Ⅰ )前胶原启动子报导基因质粒 phCAT Cα1(Ⅰ ) 2 3,phCAT Cα1(Ⅰ ) 0 8,phCAT Cα1(Ⅰ )0 4的活性 ,不能抑制α1(Ⅰ )前胶原启动子报导基因质粒 phCAT Cα1(Ⅰ ) 0 1的活性 ;(2 )TGFβ1能促进 ph CAT Cα1(Ⅰ ) 2 3的活性 ,抗纤复方能拮抗TGFβ1这一促进作用。结论 :抗纤复方能够作用于胶原α1(Ⅰ )基因调控片段 ,抑制胶原合成。 |
| 英文摘要: |
| Objective: To study the effect of Anti fibrosis Compound (AFC) on activity of human α 1(Ⅰ) precollagen gene promoter (PGP α 1) and its mechanism. Methods: Segments containing phCAT Cα 1(Ⅰ)2 3,phCAT Cα 1(Ⅰ)0 8,phCAT Cα 1(Ⅰ)0 4 and phCAT Cα 1(Ⅰ)0 1 were taken as series of targets, and chloramphenicol acetyltransferase (CAT) as reporter gene, to construct gene recombination plasmid, transform into NIH3T3, and make a stable PGP α 1 transfection cell line. It was treated by AFC and/or transforming growth factor β 1 (TGFβ 1), and the CAT activity was assessed. Results: (1) AFC could inhibit the promoter phCAT Cα 1(Ⅰ)2 3,phCAT Cα 1(Ⅰ)0 8,phCAT Cα 1(Ⅰ)0 4 activity, but showed no effect on phCAT Cα 1(Ⅰ)0 1 activity. (2)TGFβ 1 could promote the activity of phCAT Cα 1(Ⅰ)2 3,AFC could antagonize the promotion of TGFβ 1. Conclusion: AFC could specifically affect the collagen α 1(Ⅰ) gene regulation segment, inhibit the synthesis of collagen. |
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