调心方对大鼠海马脑片长时程增强效应的影响

目的 :观察调心方对大鼠海马脑片长时程增强 (LTP)效应的影响。方法 :采用细胞外微电极技术 ,记录大鼠海马脑片CA1区细胞外群峰电位 (PS) ,然后施以 10 0Hz、10 0串的强直刺激 ,诱发LTP产生。结果 :在正常情况下 ,大鼠海马脑片LTP的诱发成功率约为 6 0 % ,施以高频串刺激后PS幅度显著增高 ,PS潜伏期明显缩短。用调心方 (7 4 5mg/ml)预先孵育海马脑片对正常PS的形状、幅度均无明显影响 ,提示其并不影响海马脑片的基础突触传递 ;但施以高频刺激后脑片LTP的诱发率呈现升高趋势 ,同时其PS增幅与正常对照组比较也显著提高。结论 :调心方可提高大鼠海马脑片LTP的诱发率及增幅 ,这可能是其改善海马学习记忆功能的作用机制之一

英文摘要:

Objective: To investigate the effect of Tiaoxin Recipe (TXR) on long term potentiation in hippocampal slices of rats. Methods: Population spikes (PS) in CA 1 area of rats hippocampal slices were evoked by extracellular microelectrode recording technique, and tetanic stimulation (100Hz, 100 pulses) was used to induce long term potentiation (LTP). Results: The successful rate of evoking LTP was about 60% under normal condition, the PS amplitude increased after stimulation, whereas PS latent period decreased obviously. Pre incubation of hippocampal slices with TXR (7.45 mg/ml) showed little effect on either the shape or amplitude of PS, indicating it didn′t influence the basal synaptic transmission. However, after tetanus, the LTP induction rate and PS amplitude were significantly enhanced compard with the control. Conclusion: TXR facilitates LTP induction rate and PS amplitude in rat hippocampal slices, which might be one of the mechanisms of TXR in improving learning and memory function of hippocampus.

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