| 董庆华,郑树,吕庆华,何立明.浙江蝮蛇毒诱导人白血病Jurkat细胞凋亡的体外实验研究[J].中国中西医结合杂志,2002,(11):851-853 |
| 浙江蝮蛇毒诱导人白血病Jurkat细胞凋亡的体外实验研究 |
| Effect of Snake Venom of Agkistrodon Halys Pallas in Inducing Human Leukemic Jurkat Cell Apoptosis |
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| DOI： |
| 中文关键词: 浙江蝮蛇毒 人白血病Jurkat细胞 凋亡 Bcl-2蛋白表达 |
| 英文关键词:snake venom of Agkistrodon halys Pallas human leukemia Jurkat cell apoptosis Bcl 2 protein expression |
| 基金项目:浙江省卫生厅资助课题 (No .2 0 0 2A1 0 6);浙江省科委资助课题(No.2 0 0 2D30 0 7) |
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| 中文摘要: |
| 目的 :研究浙江蝮蛇毒诱导人白血病Jurkat细胞凋亡的作用及机理。方法 :采用MTT法测定IC50 值及生长曲线 ,流式细胞仪 (FCM )AnnexinⅤFITC/PI法检测细胞凋亡发生率 ,PI染色法检测细胞周期 ,同时用流式细胞汁FCM及Western blot检测Bcl 2蛋白表达。结果 :浙江蝮蛇毒能抑制Jurkat细胞生长 ,且呈剂量依赖关系 ,并能诱导人白血病Jurkat细胞凋亡 ,4 8h时作用最强 ,此时Bcl 2蛋白表达下降 ,随后此作用减弱 ,低浓度作用下细胞逐渐恢复生长。结论 :浙江蝮蛇毒能诱导人白血病Jurkat细胞凋亡 ,且呈剂量依赖关系 ,此作用与Bcl 2蛋白表达下调有关 |
| 英文摘要: |
| Objective: To study the effect and mechanism of snake venom of Agkistrodon Halys Pallas in Zhejiang in inducing human leukemic Jurkat cell apoptosis. Methods: IC 50 and growth curve of Jurkat cells were detected by MTT; percentage of apoptotic cells was analyzed by flow cytometry (FCM) using Annexin Ⅴ FITC/PI staining method; cell cycle was also detected by FCM and protein expression of Bcl 2 gene was detected by FCM and Western blot. Results: Zhejiang viper venom could inhibit the growth of Jurkat cells dose dependently, induce the cell apoptosis, with the Bcl 2 protein expression down regulated. The strongest effect was shown at 48 hrs, it weakened gradually, and the Jurkat cells restored slowly in low concentration of snake venom. Conclusion: Zhejiang viper venom could induce apoptosis of human leukemic Jurkat cell in dose dependent manner, the effect is related to the down regulation of Bcl 2 protein expression. |
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