川芎嗪注射液对庆大霉素耳中毒豚鼠耳蜗螺旋神经节HSP70表达的影响

Effect of Ligustrazine Injection on Gentamicin Ototoxicity Induced Heat Shock Protein 70 Expression in Cochlear Spiral Ganglion Cells of Guinea Pigs

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DOI：

中文关键词: 川芎嗪注射液庆大霉素热休克蛋白耳蜗螺旋神经节

英文关键词:ligustrazine injection gentamicin heat shock protein cochlea spiral ganglion

基金项目:

作者	单位
倪月秋	中国医科大学听力研究室沈阳110001
汤浩	中国医科大学听力研究室沈阳110001

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中文摘要:

目的:研究川芎嗪注射液(四甲基吡嗪,TMP)对庆大霉素(GM)耳中毒豚鼠耳蜗螺旋神经节热休克蛋白70(HSP70)表达的影响,探讨TMP对GM耳毒性损伤的保护作用。方法:应用SABC免疫组化技术、原位杂交技术及图像分析技术并结合听性脑干反应(ABR)测试,观察TMP对GM耳中毒豚鼠ABR阈值和耳蜗螺旋神经节HSP70和HSP70mRNA表达的影响。结果:TMP加GM组耳蜗螺旋神经节HSP70及HSP70mRNA的表达和ABR阈值明显低于GM组(P<0.01)。结论:TMP可降低耳蜗螺旋神经节HSP70及HSP70mRNA的表达,以减轻GM的耳毒性损伤,从而改善听功能。

英文摘要:

To explore the protective role of ligustrazine (tetramethylpyrazine, TMP) on gentamicin oto-toxicity. Methods: Using immunohistochemistry staining, in situ hybridization histochemistry and image quantitative analysis system, combined with auditory brainstem response (ABR) to observe the effects of TMP on threshold of ABR and the heat shock protein 70 (HSP70) and HSP70mRNA expression in cochlear spiral ganglion of gentamicin intoxicated guinea pigs. Results; The ABR threshold, and HSP70 and HSP70mRNA expression in cochlear spiral ganglion cells of gentamicin intoxicated guinea pig significantly increased, which could be reduced significantly after treatment of TMP, P<0.01. Conclusion; TMP could reduce the heightened HSP70 and HSP70mRNA expression in cochlear spiral ganglion cells induced by gentamicin to alleviate its oto-toxicity so as to improve hearing function.

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