黄芪、当归对血管平滑肌细胞粘着斑激酶表达和细胞凋亡的影响

Effect of Astragalus membranaceus and Angelica sinensis on Focal Adhesion Kinase Expression and Apoptosis of Cultured Vascular Smooth Muscle Cells

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DOI：

中文关键词: 黄芪当归粘着斑激酶血管平滑肌细胞细胞凋亡

英文关键词:Astragalus membranaceus Angelica sinensis focal adhesion kinase vascular smooth muscular cells cell apoptosis

基金项目:国家自然科学基金(No.3997074);河北省自然科学基金(No。301358)

作者	单位
杨长春	河北医科大学基础医学研究所生化研究室石家庄 050017
温进坤	河北医科大学基础医学研究所生化研究室石家庄 050017
韩梅	河北医科大学基础医学研究所生化研究室石家庄 050017

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中文摘要:

目的:探讨黄芪、当归对体外培养的血管平滑肌细胞（VSMC）粘着斑激酶（FAK）表达和细胞凋亡的影响。方法:应用RT-PCR、Western blot检测黄芪、当归对FAK表达的影响;应用 Greiss试剂检测 VSMC培养液中NO₂^-含量;应用流式细胞仪检测细胞凋亡及bcl-2和caspase-3表达活性。结果:黄芪、当归注射液可以显著抑制由碱性成纤维细胞生长因子所诱导的FAK表达,增加细胞培养液中的NO₂^-含量,诱导cas-pase-3表达。下调bcl-2表达,促进体外培养的VSMC凋亡。结论:黄芪、当归诱导VSMC凋亡与其促进NO合成、抑制FAK表达、诱导促凋亡基因caspase-3表达及下调抗凋亡基因bcl-2表达有关。

英文摘要:

Objective: To explore the effect of Astragalus membranaceus (Ast) and Angelica sinensis (Ang) on focal adhesion kinase (FAK) expression and apoptosis of cultured vascular smooth muscle cells (VSMC). Methods: RT-PCR and Western blot were used to analyze the changes of FAK expression, Greiss reagent was used to measure concentration of nitric oxide (NO) in culture medium, bcl-2 and caspase-3 protein expressions and VSMC apoptosis were detected by flow cytometry. Results: Ast and Ang could significantly inhibit FAK expression induced by basic fibroblast growth factor (bFGF), increase the NO2- content in the medium, up-regulate caspase-3 expression and down-regulate bcl-2 expression as well as induce VSMC apoptosis. Conclusion: Ast and Ang could induce VSMC apoptosis by increasing NO content, inhibiting FAK expression, down-regulating apoptotic gene bcl-2 expression and up-regulating apoptogenous gene caspase-3.

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