心脉通对家兔颈动脉内皮损伤后野生型p53基因表达的影响

目的:探讨中药复方制剂心脉通防治经皮腔内冠状动脉成形术后再狭窄作用机理。方法:用Fishman空气干燥法,建立家兔颈动脉内皮损伤模型,以组织原位杂交方法观察心脉通对家兔颈动脉内皮损伤后p53基因表达的影响。结果:术后3天出现表达,7天表达增强,14天表达高峰,21天表达减弱,28天仍然持续表达。其中A组(心脉通组)表达信号最强,B组(华法令组)弱于A组,C组(手术对照组)显著弱于A、B组。结论:心脉通能促进家兔颈动脉内皮损伤后野生型p53基因高表达,这可能是心脉通防治再狭窄的重要机理之一。

英文摘要:

Objective: To explore the mechanism of prevention and treatment of post-PTCA restenosis with Xin-maitong (XMT). Methods: Rabbit carotid endothelial injury model was established using Fishman air drying method. Effect of XMT on model rabbits wild-type p53 gene expression was observed by tissue in situ hybridization. Results: p53 gene expression appeared on the 3 days after operation, enhanced on the 7 days, reached the peak on the 14th day, weakened on the 21th, and still showed on the 28th day. The strongest expression was shown in rabbits treated by XMT, second in those treated by Warfarin, and the weakest in the operated control group. Conclusion: XMT could promote the high expression of wild-type p53 gene expression in rabbit with carotid endothelial injury, which is possibly one of the important mechanism of XMT in preventing and treating arterial restenosis.

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