| 蒋红梅,李胜富,李幼平,张立,蔡美英,吴大蓉.缺氧再给氧对内皮细胞免疫功能的影响及益生注射液干预效果初探[J].中国中西医结合杂志,2003,(6):451-454 |
| 缺氧再给氧对内皮细胞免疫功能的影响及益生注射液干预效果初探 |
| Influence of Anoxia/reoxygenation on Immunofunction of Endothelial Cells and Effect of Intervention with Yisheng Injection on It |
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| DOI： |
| 中文关键词: 内皮细胞 缺氧再给氧损伤 益生注射液 免疫学 |
| 英文关键词:endothelial cell anoxia/regeneration injury Yisheng injection immunology |
| 基金项目:国家自然科学基金(No.39993430);纽约中华医学基金会(CMB)资助(2001-03-01) |
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| 中文摘要: |
| 目的:探讨缺血再灌注(缺氧再给氧)对内皮细胞免疫功能的影响及益生注射液的干预效果。方法:用人脐静脉内皮细胞株ECV304缺氧再给氧模型模拟体内缺血再灌注对血管内皮细胞的损伤,并用益生注射液干预其缺氧再给氧过程。间接免疫荧光染色显示胞内核因子-κB(NF-κB),流式细胞术检测细胞表面HLA-ABC、HLA-DR、CD86和CD54,并将内皮细胞与同种异体淋巴细胞混合培养(MELR),检测淋巴细胞增殖程度、IL-2的产生及凋亡淋巴细胞百分率。结果:缺氧再给氧使ECV304细胞变圆、收缩、脱落,胞浆NF-κB表达增高,且由胞浆阳性为主转为胞核阳性为主,细胞表面HLA-ABC、HLA-DR、CD86增高,CD54无显著变化,MELR中~3H-TdR掺入量、IL-2生成量显著增多,凋亡淋巴细胞百分率降低。益生注射液干预后ECV304细胞形态基本恢复正常,NF-κB表达未下调,但核阳性细胞百分率降低,以核浆阳性为主,其他多项检测指标的改变得以逆转。结论:缺氧再给氧影响了ECV304细胞几种重要免疫相关分子的表达,益生注射液可发挥拮抗作用,使内皮细胞保持相对正常的免疫学功能。 |
| 英文摘要: |
| Objective: To explore the influence of ischemia/reperfusion (anoxia/reoxygenation) on immunofunction of endothelial cells (ECs) and effect of intervention with Yisheng injection (YSI, a pure natural medicine) on it. Methods: Model of ECs induced by anoxia/reoxygenation was established to mimic ECs ischemia/reperfusion injury in vivo with human umbilical vein endothelial cell line ECV304. Then YSI was used to intervene the anoxi-a/reoxygenation process. Nuclear transcriptional factor-kB (NF-kB) was exhibited by fluorescent staining, HLA-ABC,HLA-DR,CD86 and CD54 were detected by flow cytometry. Mixed endothelial cell-lymphocyte reaction (MELR) was conducted to examine the proliferation of lymphocyte, production of IL-2 and percentage of apoptotic lymphocyte. Results: Anoxia/reoxygenation made the ECV304 cell became round, shrunk and abscised, with increased plasma NF-kB, and changed from positive cytoplasm to positive nucleus. HLA-ABC, HLA-DR and CD86 on surface of cells increased but CD54 showed unchanged. MELR showed the incorporation of 3H-TdR and production of IL-2 increased significantly and the percentage of apoptotic lymphocyte decreased. After YSI intervention, the ECV304 cell shaped recovered, NF-icB expression didn’t down-regulated, but the percentage of positive cells decreased, changed to positive dominant. Besides, reversal changes were shown in other parameters. Conclusion: Anoxia/reoxygenation influences some important immune related molecules in ECV304 cells, YSI could antagonizing these influences to maintain the immune function of endothelial cells in a relative normal manner. |
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