骨碎补对骨愈合过程中相关基因表达的影响

作者	单位
董福慧	中国医学科学院中国协和医科大学医药生物技术研究所病毒室北京 100050
郑军	中国医学科学院中国协和医科大学医药生物技术研究所病毒室北京 100050
程伟	中国医学科学院中国协和医科大学医药生物技术研究所病毒室北京 100050

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中文摘要:

目的:观察中药骨碎补对骨愈合过程中相关基因表达的影响。方法:通过在大鼠胫骨打孔的方法建立单因素干扰模型,在不同时间点,采用原位杂交方法检测骨愈合过程中Ⅰ、Ⅱ、Ⅲ型前胶原mRNA、转化生长因子（TGF）-β₁mRNA、骨形态发生蛋白（BMP）-2mRNA及血管内皮生长因子（VEGF）mRNA表达的动态观察。结果:Ⅰ、Ⅱ、Ⅲ型前胶原mRNA、TGF-β₁mRNA、BMP-2mRNA及VEGFmRNA在正常组各时间点均无阳性表达;模型组在不同时间点的不同细胞中表达强度不同;与模型组比较骨碎补组在TGF-β₁mRNA、BMP-2mRNA和Ⅰ型前胶原mRNA的表达上有显著变化,骨碎补组不同基因的作用不同,作用时间点不同,作用强度不同。结论:在骨愈合过程中,骨碎补对TGF-β₁mRNA、BMP-2mRNA基因表达具有有益的调节作用。

英文摘要:

Objective: To explore the influence of Rhizoma Drynariae (RD) in intervening the process of bone repairing, understanding its regulatory target to seek for the pathway of TCM genomics creation, through observing dynamically the changes of mRNAs of procollagen Ⅰ, Ⅱ and Ⅲ, transforming growth factor B1(TGFB1), bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF). Methods: Single factor interference model of bone injury was set up in SD rat by means of tibia drilling, and the changes of above-mentioned mRNAs in different times in the model animal were observed by adopting situ hybridization method. Results: All the mRNAs in the normal group showed no significant positive expression at various time points. The expression intensity of mRNAs revealed in the model group varied depending on the time and the kind of cells, the changes in the RD treated group were higher than those in the untreated model group. The functional strength of RD for various genes was in different functional time points. Conclusion: In the course of promoting bone repairing, RD has favorable influence in regulating mRNAs of TGF-B1, BMP-2 and VEGF gene expression .

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