三七总皂甙对免疫介导性再生障碍性贫血小鼠造血祖细胞的增殖作用

作者	单位
盖云	上海市第七人民医院中医科
高瑞兰	浙江中医学院附属医院
牛泱平	浙江中医学院附属医院
金锦海	浙江中医学院附属医院
史亦谦	浙江中医学院附属医院

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中文摘要:

目的 :探讨三七总皂甙 (PNS)对免疫介导性再生障碍性贫血 (再障 )小鼠模型血细胞生成的作用。方法 :采用免疫介导法进行再障造模 ,Balb/c小鼠经6 0 Coγ射线亚致死量照射后 ,自尾静脉输入DBA/ 2小鼠的淋巴细胞 ,随机分成 4组 ,即模型组、PNS治疗高、中和低剂量组 (每只 3 2、1 6、0 8mg/d) ,并设正常对照组。腹腔注射给药 ,模型组和正常对照组注射生理盐水。治疗 12天后 ,做血白细胞计数、骨髓病理检查和粒系、红系造血祖细胞 (CFU GM、CFU E)集落培养。结果 :(1)PNS升高白细胞数 ,与模型组比较 ,PNS高、中和低剂量组的白细胞数提高率分别为 (34 3± 2 9) % ,(2 9 2± 1 7) %和 (14 5± 1 6 ) % (P <0 0 1或P <0 0 5 )。 (2 )改善骨髓抑制 ,骨髓病理检查模型组出现大片空白区 ,被大量的脂肪组织代替 ,而PNS治疗组造血组织结构较完整 ,造血细胞量丰富。 (3)促进造血祖细胞增殖 ,高、中和低剂量PNS对CFU GM集落提高率分别为 (6 4 4± 2 8) %、(6 7 3± 2 4 ) %和 (2 1 9± 1 8) % (均P <0 0 1) ;CFU E集落的提高率分别为(31 9± 3 6 ) %、(2 0 7± 2 4 ) %和 (12 8± 2 6 ) % (P <0 0 1或P <0 0 5 )。结论 :免疫介导性再障小鼠在骨髓抑制、造血功能低下时 ,PNS通过促进骨髓粒系、红系造血祖

英文摘要:

Objective: To study the effect of panax notoginsenosides (PNS) on the proliferation of hematopoietic progenitor cells (HPC) in mice with immune-mediated aplastic anemia. Methods: Balb/c mice model of immune-mediated aplastic anemia was established by radiation with sublethal dose of 60 Co following the intravenously infusing lymphocytes of DBA/2 mice. Model mice in the treated groups were treated separately with high, middle and low dose of PNS, 3.2mg, 1.6 mg and 0.8 mg per day respectively by intraperitoneal injection. Model mice in the control group and normal mice in the normal control group were treated with normal saline. The peripheral white blood cell (WBC) count and pathological examination of bone marrow were carried out 12 days later, the bone marrow was taken to be incubated in semi-solid culture system for observing proliferation of HPC. Results: PNS could (1) increase peripheral WBC count: as compared with that in the model control, WBC in the high, middle and low dose PNS groups was raised by (34.3±2.9)%, (29 2±1 7)% and (14 5±1 6)% respectively, P<0 01 and P<0 05; (2) improve the bone marrow inhibition: pathological examination showed in the model group, the hematopoietic structure was destroyed and replaced by fatty tissue, while in the PNS treated groups, the structure of marrow was rather complete and filled with abundant hematopoietic cells; (3)promote the proliferation of HPC: as compared with the model group, the colony formation of CFU-GM were increased by (64 4±2 8)%, (67.3±2.4)% and (21.9±1.8)% respectively and that of CFU-E increased by (31.9±3.6)%, (20.7±2.4)% and (12.8±2.6)% respectively in the three PNS treated group (P<0 01 and P<0 05). Conclusion: PNS could enhance hematopoiesis by promoting proliferation of CFU-GM and CFU-E progenitors so as to improve the hematopoietic function in mice of immune-mediated aplastic anemia.

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