| 李平,李培恒,张子义,李克明,陈钢.柴苓汤及其有效成分对大鼠实验性肾炎的影响[J].中国中西医结合杂志,2003,(10):757-759 |
| 柴苓汤及其有效成分对大鼠实验性肾炎的影响 |
| Effect of Chailing Decoction and Its Active Ingredients on Experimental Nephritis in Rats |
| 免费下载全文 查看/发表评论 下载PDF阅读器 |
| |
| DOI： |
| 中文关键词: 单克隆抗体 蛋白尿 柴苓汤 柴胡皂甙-d |
| 英文关键词:monoclonal antibody proteinuria Chailing decoction saikosaponin-d |
| 基金项目:国家自然科学基金资助课题 (No .30 2 71 52 0 );国家中医药管理局资助课题 (2 0 0 0 J p 64) |
|
| 摘要点击次数: 1340 |
| 全文下载次数: 1899 |
| 中文摘要: |
| 目的 :筛选柴苓汤治疗大鼠肾小球肾炎模型的有效成分。方法 :采用注射单克隆抗体 1 2 2 3造成大鼠肾小球肾炎模型 ,进行 5次连续的实验筛选柴苓汤中的有效成分。每次实验选用 2 8只模型大鼠 ,随机分为 4组 ,每组 7只。第 1组用PBS作对照 ,第 2、3、4组分别给予柴苓汤及其不同的成分 ,于造模前 5天及造模后持续 8天腹腔注射给药 ,共给药 13天。造模后第 8天处死全部大鼠 ,观察不同药物对大鼠蛋白尿、肾脏形态学改变以及血生化指标的影响。结果 :柴苓汤组、小柴胡汤组、中药柴胡的不同组合及其成分———柴胡皂甙 d ,可以抑制蛋白尿和大鼠肾小球系膜细胞的增生以及细胞外基质的扩张。结论 :柴苓汤和其有效成分对单克隆抗体 1 2 2 3引起的大鼠肾小球肾炎模型有抑制作用 ,其有效单体成分为柴胡皂甙 d。 |
| 英文摘要: |
| Objective: To screen the active ingredients of Chailing decoction (CLD) by using rat nephritis model induced by mono-colonal antibody 1-22-3 (mAb) injection. Methods: The active ingredients of CLD was screened by 5 successive times of experiment. In each time, 28 rats were randomly divided into 4 groups, 7 in each. Group 1 was treated with PBS as control, Groups 2-4 were treated separately with CLD and its various ingredients, the medication was started 5 days before and lasted to 8 days after modeling by peritoneal injection, 13 days totally. All the rats were killed 8 days after modeling to observe the effect of various drugs on proteinuria, morphological change of kidney and biochemical parameters. Results: CLD, Xiaochaihu decoction, various combination of thorowax root and its extract (saikosaponin-d) could reduce urinary protein, inhibit the proliferation of mesangial cell and expansion of extracellular matrix. Conclusion: CLD and its active ingredients had inhibition on mAb induced rat model of nephritis, the active is saikosaponin-d. |
| 关闭 |
|
|
|
扫描微信二维码，获取更多信息