大黄虫丸对大鼠肝星状细胞表达TGF-β<sub>1</sub>的影响

作者	单位
李岚生	珠海市人民医院暨南大学医学院第三附属医院中医病区
徐曼	重庆医科大学病理学教研室
刘志龙	广东省中医院珠海医院
邓江华	珠海市人民医院暨南大学医学院第三附属医院中医病区

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中文摘要:

目的 :探讨大黄虫丸对大鼠肝星状细胞表达转化生长因子β1(TGF β1)的影响。方法 :用CCl4复合因素致大鼠肝纤维化模型 ,同时分别给予 3种剂量大黄虫丸治疗 ,免疫组化方法观察TGF β1、α 平滑肌肌动蛋白 (α SMA)的合成表达及纤维化程度分级。结果 :经给予大黄虫丸干预治疗常规剂量组大鼠TGF β1、α SMA表达与模型组比较差异无显著性 (P >0 0 5 ) ,但双倍剂量组和三倍剂量组TGF β1、α SMA在汇管区及不连续纤维间隔的表达明显减弱 (P <0 0 5 ,P <0 0 1)。结论 :大黄虫丸较大剂量能抑制肝星状细胞增殖和分泌TGF β1,减少胶原生成 ,从而减弱肝星状细胞的自分泌放大效应 ,这可能是大黄虫丸抗肝纤维化作用的主要机制之一。

英文摘要:

Objective: To explore the effect of Dahuang Zhechong Pill (DHZCP) on transforming growth factor-β 1 (TGF-β 1) in rats hepatic stellate cells. Methods: Liver fibrosis model rats were induced by CCl 4 compound factor, and treated with DHZCP in three different dosages (ordinary, double and triple) separately. TGF-β 1 and α-smooth muscle actin (α-SMA) synthesis expression, and grades of fibrosis were observed. Results: TGF-β 1 and α-SMA expression in the group treated with ordinary dose of DHZCP was insignificantly different from those in the control group (P>0.05), but the expression attenuated significantly after treatment with double or triple dose of DHZCP in the portal area and discontinuous fibrous septum (P<0.05, P<0.01). Conclusion: DHZCP of larger dosage could inhibit the hepatic stellate cells proliferation and secretion of TGF-β 1, and reduce the genesis of collagen, so as to weaken the auto-secretion amplifying response of the cells, which might be one of the chief mechanisms of DHZCP in antagonizing liver fibrosis.

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