当归芍药散加味方及其拆方对中老年大鼠淋巴细胞增殖的影响

作者	单位
何宏文	广州中山大学中山医学院人体解剖学教研室脑研究室广州 510089
王传恩	广州中山大学中山医学院人体解剖学教研室脑研究室广州 510089
谢瑶	广州中山大学中山医学院人体解剖学教研室脑研究室广州 510089
姚志彬	广州中山大学中山医学院人体解剖学教研室脑研究室广州 510089

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中文摘要:

目的:研究当归芍药散加味方(DSS)及其拆方对中老年大鼠淋巴细胞功能的影响。方法:选用中老年大鼠(18月龄),分组灌服DSS全方及其拆方水煎液,3周后,各组大鼠脾细胞分别加入ConA、异丙肾上腺素(ISO)、心得安或DSS大鼠血清诱导淋巴细胞增殖,用四氮唑蓝比色法比较各组大鼠淋巴细胞增殖(A值)变化。结果:全方组、君臣药组、佐药组大鼠淋巴细胞增殖的A值与对照组比较,差异有显著性(P<0.01);加入ISO后全方组各ConA浓度A值明显减少(P<0.01),再加入心得安或DSS大鼠血清后,A值又恢复到未加ISO水平。结论:DSS及其拆方均可促进老年大鼠淋巴细胞增殖,DSS可拮抗ISO的抑制淋巴细胞增殖作用。

英文摘要:

Objective: To study the effect of supplemented Danggui Shaoyao San (DSS) and its disassembled prescriptions on function of lymphocyte in aged rats. Methods: Rats aged 18 months were administered with decoction of DSS and its disassembled prescriptions separately in groups for 3 weeks to prepare drug containing serum. Samples of ConA induced rats splenic lymphocytes proliferation was treated respectively with prepared drug serum, for testing their effect on lymphocyte proliferation using methyl thiazolyl tetrazolium (MTT) method, and isoprel (ISO) and propranolol were taken as the controls. Results: The A value of lymphocyte proliferation in the drug serum treated groups was significantly different from that in the control groups (P<0.01), which could be reduced markedly after treatment with ISO, but could restore to the level before ISO treatment by adding drug serum or propranolol. Conclusion: DSS and/or its disassembled prescriptions could raise the lymphocyte proliferation in aged rats significantly, it also shows antagonizing effect against the inhibition of ISO on lymphocyte proliferation.

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