栝蒌根提取物对Hela细胞作用的初步研究

目的:研究栝蒌根提取(简称栝蒌根)物体外对Hela细胞毒作用,探讨其抑癌作用机理。方法:用MTT法检测栝蒌根对Hela细胞毒作用,扫描电镜和透射电镜观察细胞超微结构变化,DNA琼脂糖凝胶电泳法检测其生化特征改变。结果:Hela细胞经栝蒌根作用24～48h后,细胞体外杀伤作用明显,呈量效、时效关系(r>0.880,P<0.01);电镜观察可见Hela细胞表面微绒毛明显减少或消失,胞膜发泡,细胞核固缩,核染色质浓缩边集,并出现凋亡小体;细胞DNA经琼脂糖凝胶电泳可见典型的梯形带。结论:栝蒌根提取物对Hela细胞具有良好的抗癌活性,其作用机制与诱导细胞凋亡相关。

英文摘要:

Objective: To investigate the cytotoxic effect of extracts of Trichosanthes kirilowi (TK) root on Hela cells in vitro and its probable anti-tumor mechanism. Methods: The cytotoxic effect in vitro on the growth of Hela cells was evaluated by microculture tetrazolium assay (MTT). Cell ultrastructural changes were examined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and DNA agarose elec-trophoresis was performed to determine apoptosis and biochemical changes of Hela cells. Results: Exposure of Hela cells to TK extracts for 24 - 48 hrs resulted in a cell growth arrest, which showed in a time- and dose-dependent manner (r>0.880, P<0.01). With SEM and TEM, marked changes were observed, including mi-crovilli disappearance or reduction, cell membrane vesiculation, cell shrinkage, condensation of chromosomes and apoptotic bodies with complete membrane. Besides, the apoptosis of Hela cells was confirmed by typical DNA ladder formation on gel electrophoresis. Conclusion: Extracts of TK has a marked anti-tumor activity and could induce apoptosis of Hela cells.

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