枸杞子、淫羊藿对衰老-年轻2BS融合细胞DNA合成的影响

作者	单位
吴白燕	北京大学医学部细胞生物学与遗传学系
邹俊华	北京大学医学部细胞生物学与遗传学系
孟书聪	北京大学医学部细胞生物学与遗传学系
郑振	复旦大学华山医院

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中文摘要:

目的 :研究枸杞子、淫羊藿水提液对衰老 -年轻 2BS融合细胞DNA合成的影响。方法 :以人胚肺二倍体成纤维细胞国内标准株 2BS为衰老模型 ,用细胞脱核和细胞融合技术 ,观察枸杞子、淫羊藿对衰老-年轻 2BS融合细胞的DNA合成的影响。结果 :2BS细胞分别在含 0 0 2 5 (V/V)枸杞子、淫羊藿水提液的DMEM培养液中 ,可连续培养至 6 1 0± 2 9、5 6 0± 2 6代 ,对照组为 4 9 0± 2 6代 (P <0 0 1) ;衰老的 2BS细胞分别用枸杞子、淫羊藿水提液处理 2h后 ,进行细胞脱核并与年轻 2BS细胞融合 ,其〔3H〕TdR掺入百分率明显高于对照组 (P <0 0 1)。结论 :枸杞子、淫羊藿均能促进衰老 -年轻 2BS融合细胞的DNA的合成速率 ,延长 2BS细胞寿命。

英文摘要:

Objective: To study the effect of water extracts of Wolfberry fruit (WB) and Epimedium (EM) on DNA synthesis of the aging-youth 2BS fusion cells. Methods: Human embryonic lung diploid fibroblasts 2BS national standard strain, were used as an aging model. Cell denucleation and cell fusion techniques were applied to observe the effect of WB and EM on DNA synthesis of 2BS fusion cells. Results: In the 0.025 (V/V) WB or EM water extract containing media, 2BS cells could be continuously cultured for 61.0±2.9 passages and 56.0±2.6 passages respectively, while in the control group it was only 49.0±2.6 passages (P<0.01). After treatment with WB and EM separately for 2 hrs, the aging 2BS cells were denucleated and fused with young 2BS cells. The 〔 3H〕TdR incorporation percentage in these treated cells was significantly higher than that in the untreated control cells (P<0.01). Conclusion: Both WB and EM can accelerate the DNA synthesis rate of the aging-youth 2BS fusion cells and prolong the life span of 2BS cells.

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