抗纤复方药物血清对HSC-LI90细胞Ⅰ型、Ⅳ型前胶原和基质金属蛋白酶及其组织抑制因子-1基因表达的影响

Effect of Anti-fibrosis Compound Contained Serum on Procollagen Type Ⅰ and Ⅳ, Matrix Metalloproteinase and Its Tissue Inhibitor-1 Gene Expression in HSC-LI90 Cell Line

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DOI：

中文关键词: 抗纤复方肝星形细胞 Ⅰ型前胶原 Ⅳ型前胶原金属蛋白酶类组织抑制因子 Northern印迹杂交酶图法

英文关键词:Anti-Fibrosis Compound Recipe liver stellate cell procollagen type Ⅰ procollagen type Ⅳ matrix metalloproteinase tissue inhibitor Northern blot hybridization zymography

基金项目:国家自然科学基金资助课题 (No .39670 90 6)

作者	单位
赵钢	上海中医药大学附属曙光医院肝科上海200021
王灵台	上海中医药大学附属曙光医院肝科上海200021
陈建杰	上海中医药大学附属曙光医院肝科上海200021
张斌	上海中医药大学附属曙光医院肝科上海200021

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中文摘要:

目的 :探讨抗纤复方药物血清对肝星形细胞 (HSC)LI90细胞 (HSC LI90 )Ⅰ型、Ⅳ型前胶原和基质金属蛋白酶 (MMPs)及其组织抑制因子 (TIMP 1)基因表达的影响。方法 :以 0 5、2 0、4 0 g/kg等不同剂量抗纤复方灌胃大鼠 ,制备药物血清 ,作用于HSC LI90细胞 4 8h ,应用Northern印迹杂交的方法 ,检测Ⅰ型、Ⅳ型前胶原、基质金属蛋白酶 2 (MMP 2 )及膜型基质金属蛋白酶 (MT1 MMP)及其组织抑制因子(TIMP 1)基因的表达 ,并用酶图法检测基质金属蛋白酶 2的活性。结果 :(1)抗纤复方不同浓度药物血清能抑制HSC LI90细胞Ⅰ型、Ⅳ型前胶原及其组织抑制因子TIMP 1的基因表达 (P <0 0 5或P <0 0 1) ;(2 )能增加膜型基质金属蛋白酶基因表达 (P <0 0 1) ;(3)对基质金属蛋白酶 2基因表达及活性无影响 (P >0 0 5 )。结论 :(1)抗纤复方具有抗肝纤维化作用 ;(2 )抗纤复方抑制HSC LI90细胞TIMP 1基因表达水平 ,促进胶原降解 ,可能是抗肝纤维化的作用机制之一。

英文摘要:

Objective:To study the effects of Anti-fibrosis Compound contained serum (AFCS) on procollagen type Ⅰ and Ⅳ (ProC-Ⅰ and ProC-Ⅳ), matrix metalloproteinase (MMP) and its tissue inhibitor (TIMP-1) gene expression in hepatic stellate cell line LI90 (HSC-LI90). Methods:AFCS was prepared by gastric infusing different dosage (0.5g/kg, 2.0 g/kg and 4.0 g/kg) of Anti-fibrosis Compound Recipe to rats. After HSC-LI90 cells were exposed to AFCS for 48 hrs, levels of ProC-Ⅰ, ProC-Ⅳ, gene expression of MMP-2, MMP membrane type 1 (MT1-MMP) and TIMP-1 in the cells were detected by Northern blot, and gelatinase activity of MMP-2 was measured by zymography. Results:AFCS of different concentrations could inhibit ProC-Ⅰ and ProC-Ⅳ and TIMP-1 gene expression (P<0.05 or P<0.01), increase MT1-MMP gene expression (P<0.01), but it showed no effect on gene expression and activity of MMP-2 (P>0.05).Conclusion:Anti-fibrosis Compound Recipe has anti-liver fibrosis action, its effects in inhibiting TIMP-1 gene expression of HSC-LI90 cells and promoting degradation of collagen might be one of the mechanisms of the action.

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