| 王悦芬,王彦刚,李恩,孔德娟.补肾方药对去卵巢大鼠端脑、海马晚期糖化终末产物及β-淀粉样肽含量的影响[J].中国中西医结合杂志,2004,(3):247-250 |
| 补肾方药对去卵巢大鼠端脑、海马晚期糖化终末产物及β-淀粉样肽含量的影响 |
| Effects of Tonifying Shen Recipe on Advanced Glycation End Products, Amyloid-β Peptide in Telencephalon and Hippocampus of Ovariectomized Rats |
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| DOI： |
| 中文关键词: 补肾方药 卵巢切除 学习记忆 晚期糖化终末产物 β-淀粉样肽 |
| 英文关键词:Tonifying Shen recipe ovariectomy learning and memory advanced glycation end products amyloid-β peptide |
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| 摘要点击次数: 1345 |
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| 中文摘要: |
| 目的 探讨补肾方药对去卵巢大鼠端脑、海马晚期糖化终末产物(AGEPs)及β-淀粉样肽(AβP)含量的影响。方法 将30只9月龄SD雌性大鼠随机分为假手术组、去卵巢组和补肾方药组。于卵巢切除4周后灌胃给药,连续治疗16周,分别用竞争性ELISA法、放免法检测端脑、海马AGEPs及AβP的含量;荧光法检测血清、尿可溶性AGE-肽的含量;并观察大鼠行为学及海马形态学变化。结果 与假手术组比较,去卵巢组逃避正确率明显降低(P<0.01),端脑AβP、血清可溶性AGE-肽的含量明显升高(P<0.05);端脑、海马AGEPs含量有升高的趋势,但无统计学意义;嗜银染色可见神经纤维增粗,大脑皮质颞叶出现老年斑。补肾方药组端脑AGEPs、AβP含量及海马AβP含量明显减少(P<0.05),尿中可溶性AGE-肽明显增加(P<0.05),学习记忆以及神经元变性得到明显改善。结论 补肾方药能降低端脑AGEPs的积累及端脑、海马组织的AβP含量,改善由雌激素缺乏所致的学习记忆下降,其机理可能是通过肾脏对AGE-肽排泄增加实现的。 |
| 英文摘要: |
| To explore the effect of Tonifying Shen recipe (TSR) on advanced glycation end products (AGEPs), amyloid-β peptide (AβP) in telencephalon and hippocampus of ovariectomized rats. Methods Female SD rats, 9 months old, were randomly divided into 3 groups: the sham operation (Sh) group, the ovariectomized (OV) group and the TSR group. Medication via gastrogavage 4 weeks was given after ovariectomy consecutively for 16 weeks. Competitive ELISA and radioimmunoassay (RIA) was used to detect AGEPs and AβP level in rats’ telencephalon and hippocampus; flores-cent assay was used to determine the serum and urinary soluble AGE-peptide level; and also the rats’ behavioral alteration and hippocampal morphological change were observed. Results Compared with those in the Sh group, the correct escape rate in the OV group was significantly lower (P<0.05), content of AβP in telencephalon and serum soluble AGE-peptide levels significantly higher (P<0.05) , contents of AGEPs in telencephalon and hippocampal tissue showed an increased tendency, but without significance (P>0.05), argyrophil stain showed that nerve fiber thickened, senile plaques appeared in temporal cortex. In the TSR group after treatment, contents of AGEPs in telencephalon, AβP in telencephalon and hippocampus significantly decreased (P<0.05), the urinary soluble AGE-peptide level significantly increased (P<0.05) and with markedly improvement of learning memory capability and above-mentioned pathological changes. Conclusion TSR could reduce the accumulation of AGEPs in telencephalon and the contents of AβP in telencephalon and hippocampal tissue, and improve the impaired ability of learning and memory caused by lack of estrogen. Its mechanism might be based on the elevation in excretion of AGE-peptide via kidney. |
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