补肾益气方对正常早孕期人细胞滋养层细胞生物学行为的影响

作者	单位
王海燕	复旦大学上海医学院免疫教研室上海200011
归绥琪	复旦大学上海医学院免疫教研室上海200011
徐丛剑	复旦大学上海医学院免疫教研室上海200011
储以微	复旦大学上海医学院免疫教研室上海200011

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中文摘要:

目的探讨补肾益气方对正常早孕期人细胞滋养层细胞增殖、侵袭、分化等生物学行为的影响。方法体外培养正常早孕期人细胞滋养层细胞 ,分别设空白对照组、5 %含药血清组、10 %含药血清组、2 0 %含药血清组 ,用扫描电镜、噻唑蓝 (MTT)法、流式细胞仪、Transwell侵袭实验检测含药血清作用 2 4h、4 8h、72h后细胞的形态、增殖活性及侵袭力的变化。结果含药血清培养 2 4h、4 8h、72h后 ,细胞滋养层细胞微绒毛变丰富 ,伪足增多、延长 ;细胞在酶联免疫分析仪 4 90nm波长的吸光度值增加 (P <0 0 1) ;sub G1期细胞减少 (P <0 0 1) ,S期细胞增加 (P <0 0 1) ,G2 /M期细胞明显减少 (P <0 0 1) ;每视野穿过PET膜的细胞数明显增多 (P <0 0 1)。结论补肾中药促进人细胞滋养层细胞增殖及侵袭力 ,并可能影响其分化能力

英文摘要:

ObjectiveTo investigate the effect of Chinese drugs for tonifying Shen on the bioactivity, cell proliferation, invasion and differentiation of human cytotrophoblast of early pregnancy. MethodsThe human early pregnant cytotrophoblast was cultured in vitro, and treated with saline (as blank control) and drug containing serum in different concentrations (5%, 10% and 20%) respectively. The changes of morphology, proliferation and invasive capacity of cells were examined by scanning electron microscopy, MTT method, flow cytometry, Transwell invassive assay at 24 hrs, 48 hrs and 72 hrs after treatment. ResultsAfter cells being cultured with drug containing serum, the cytotrophoblast became abundant in microvilli, with more and prolonged pseudopodia. The cell absorbency in 490nm wave length increased significantly (P<0 01), cells of sub G 1 and G 2/M phase obviously decreased and that of S phase increased (P<0 01), and the cells penetrated through PET membrane in each visual field significantly increased (P<0 01). ConclusionChinese herbs for tonifying Shen could promote the proliferation and invasive capacity of cytotrophoblast and might influence its differentiation.

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