调心方对Aβ大鼠痴呆模型脑组织IL-1β、IL-6及APPmRNA表达的影响

作者	单位
周晖	上海中医药大学基础医学院生化教研室上海200032
赵伟康	上海中医药大学基础医学院生化教研室上海200032
金国琴	上海中医药大学基础医学院生化教研室上海200032

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中文摘要:

目的探讨中药治疗Alzheimer型痴呆 (AD)的有效方剂调心方的神经免疫调节作用机制。方法采用免疫组化、RT PCR方法分别研究了侧脑室注射 β 淀粉样蛋白 (Aβ1- 40 )诱导的AD大鼠模型神经免疫学病理变化。主要包括脑组织中胶质细胞原纤维酸性蛋白 (GFAP)的表达及炎性细胞因子白细胞介素 1β(IL 1β)、白细胞介素 6 (IL 6 )mRNA和Aβ前体 βAPPmRNA基因表达水平 ,并观察调心方的作用。结果Aβ在脑组织的沉积能诱导星形胶质细胞的激活以及炎性细胞因子IL 1β、IL 6mRNA水平异常增高 ,APPmRNA水平也明显提高 ;而调心方能有效控制以上病理变化。结论调心方能有效地控制Aβ启动的炎症和免疫级联反应 ,提示有效控制AD患者脑内Aβ免疫级联反应是调心方的重要作用机制之一。

英文摘要:

ObjectiveTo investigate the neuro immune regulatory mechanism of Heart Benefiting recipe (HBR), an effective recipe for treatment of Alzheimer’s disease (AD). MethodsUsing immunohistochemical and RT PCR methods, the neuro immunological pathological changes in the AD rat model induced by β amyloid protein (Aβ1 40) via lateral cerebral ventricle injection, including mainly the glial fibrillary acidic protein expression and inflammatory cytokines IL 1β, IL 6mRNA and β amyloid protein precursor (APPmRNA) gene expression were studied. And the effects of HBR on these parameters were observed. ResultsDeposition of Aβ in cerebral tissue could induce activation of stellate glial cells and abnormal increased levels of inflammatory cytokines (IL 1β and IL 6mRNA), also the elevation of APPmRNA level. HBR could effectively control the above mentioned pathological changes. ConclusionHBR could effectively control the inflammation and the Aβ immune cascade reaction in brain of AD patients, it is one of the important therapeutic mechanisms of the recipe.

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