| 季小梅,王谦,齐昉,王跃秀,于玫,黄启福,李伯光,严京.消渴颗粒剂对糖尿病肾病大鼠血糖、尿蛋白及肾小球形态的影响[J].中国中西医结合杂志,2004,(6):534-537 |
| 消渴颗粒剂对糖尿病肾病大鼠血糖、尿蛋白及肾小球形态的影响 |
| Effect of Xiaoke Granule on Blood Glucose, Urinary Protein and Glomerular Morphology in Rats with Diabetic Nephropathy |
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| DOI： |
| 中文关键词: 消渴颗粒剂 糖尿病肾病 血糖 蛋白尿 形态学观察 |
| 英文关键词:Xiaoke granule diabetic nephropathy blood glucose proteinuria morphological observation |
| 基金项目:北京市教育委员会科技发展计划项目 (No.0 0KJ 0 74);北京市中医药管理局资助课题 (2 0 0 0年 ) |
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| 中文摘要: |
| 目的观察糖尿病肾病大鼠血糖、尿蛋白的变化以及消渴颗粒剂对其肾小球形态的影响。方法采用 3/ 4肾切除 ,腹腔一次性注射链脲佐菌素 (streptozotocin ,STZ)复制大鼠糖尿病肾病模型。将动物分为模型组、消渴颗粒剂治疗组 (中药治疗组 )、阳性药对照组和假手术组。每组大鼠于注射STZ后 1、2、3、4、5周尾尖取血测定血糖和 2 4h尿蛋白。各组大鼠于注射STZ后 2、6周分别杀检 ,进行肾脏形态学观察。结果注射STZ后 6周 ,模型组大鼠有不同程度的肾小球硬化 ,中药治疗组上述病变明显轻于模型组。注射STZ后 1、2、3、4、5周模型组血糖明显高于假手术组 (P <0 0 5 ) ,中药治疗组和阳性药对照组与模型组同期比较血糖明显下降。 2 4h尿蛋白模型组大鼠于造模后第 1、2、3、4、5周明显高于假手术组 ;中药治疗组在 1、2、3周明显低于模型组。模型组血糖和 2 4h尿蛋白呈正相关。结论采用此方法成功的复制了大鼠糖尿病肾病模型 ,糖尿病肾病大鼠血糖增高可能引起蛋白尿 ,消渴颗粒剂治疗糖尿病肾病的途径之一是降低大鼠血糖 ,减轻蛋白尿 ,从而改善肾小球病变。 |
| 英文摘要: |
| ObjectiveTo observe the changes of blood glucose, urinary protein and renal glomerular morphology in rats with diabetic nephropathy, and the effect of Xiaoke granule (XKG) on them. MethodsThe diabetic nephropathy model was established by 3/4 nephrectomy and once intraperitoneal injection of streptozotocin (STZ). The experimental rats were divided into the model group, the XKG group, the positive control group and the sham operation group. Blood was taken from rat’s caudal vein to test the fasting blood glucose (FBG) once every week after STZ injection and at the same time, urinary protein in 24 hrs (UP/24h) was investigated. All the rats were sacrificed 2, 6 weeks after STZ injection and morphological examination on their kidney was performed. ResultsSix weeks after STZ injection, glomerular sclerosis in various degrees was seen in the model group, but the pathological change was significantly milder in the treated groups. FBG in the model group was higher than that in the sham operation group at all time points respectively (P<0 05), while in the XKG group and the positive control group, the change was improved significantly. UP/24h in the model group was higher than that in the sham operation group at all time points respectively, and that in the XKG group at 1, 2 and 3 weeks after STZ injection was significantly lower than that in the model group. FBG and UP/24h showed a positive correlation. ConclusionA rat model of diabetic nephropathy was duplicated successfully. The elevated blood glucose level in diabetic nephropathy model could induce proteinuria. One of the routes of treatment of diabetic nephropathy by XKG is to reduce the blood glucose, eliminate the proteinuria, and thus to improve the pathological change in renal glomeruli. |
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