脉络宁对大鼠脑梗死C-Fos蛋白表达的影响

作者	单位
郭志力	金陵药业股份有限公司
刘平	南京军医学院组织胚胎学教研室
陈泳宏	南京军医学院组织胚胎学教研室
商舰	南京军医学院组织胚胎学教研室
张留宝	南京军医学院组织胚胎学教研室
盛春宁	南京军医学院组织胚胎学教研室
骆云	南京军医学院组织胚胎学教研室

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中文摘要:

目的探讨脉络宁对光化学诱发脑梗死后神经细胞保护作用的可能机制。方法 80只SD大鼠分为 3组 ,(1)对照组 (n =2 0 ) ;(2 )脑梗死组 (n =30 ) ;(3)脉络宁组 (n =30 )。使用光化学诱发的大鼠局灶性脑缺血模型 ,采用免疫组化、图像分析及透射电镜等方法 ,观察脉络宁治疗前后C Fos蛋白表达的变化。结果C Fos阳性细胞位于坏死中心与正常皮层之间的半影区 ,脑梗死 3h即出现C Fos蛋白表达 ,6h达到高峰。脉络宁组C Fos表达与梗死组比较明显减少 (P <0 .0 1)。结论脉络宁可明显减少半影区神经细胞的损伤 ,其对神经元的保护作用可能与抑制C Fos蛋白的表达有关。

英文摘要:

Objective To investigate the protective effect of Mailuoning (MLN) on nerve cells after cerebral infarction induced by photochemistry. Methods Eighty SD rats were divided into three groups, the control group (n=20), the model group (n=30) and the MLN group (n=30). Focal cerebro ischemia induced by photothrombosis in adult rat was used as a model. Changes of C Fos protein expression before and after MLN treatment were observed using immunohistochemistry, computerized imaging technique and transmission electron microscopy (TEM). ResultsC Fos positive cells located in the transitional zone between the necrotic core and normal cortex. C Fos protein expression began to show 3 hrs after cerebral infarction, peaked at 6 hrs. As compared with the model group, C Fos expression was significantly reduced in the MLN group (P<0 01). Conclusion MLN could markedly reduce the injury of nerve cell in the transitional zone, the protection may be related with its inhibition on C Fos protein expression.

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