川芎嗪对脂多糖诱导巨噬细胞环氧化酶-2表达和心肌细胞凋亡的影响

作者	单位
万敬员	华中科技大学同济医学院病理生理学系武汉430030
叶笃筠	华中科技大学同济医学院病理生理学系武汉430030
吴萍	华中科技大学同济医学院病理生理学系武汉430030
张力	华中科技大学同济医学院病理生理学系武汉430030
龚霞	华中科技大学同济医学院病理生理学系武汉430030
黄云峰	华中科技大学同济医学院病理生理学系武汉430030

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中文摘要:

目的观察川芎嗪对脂多糖 (LPS)诱导RAW 2 64 7小鼠巨噬细胞环氧化酶 2 (COX 2 )基因表达和活性的影响 ,进一步研究川芎嗪对脂多糖诱导乳鼠心肌细胞凋亡的作用及其机制。方法采用RT PCR和免疫印迹检测巨噬细胞COX 2基因表达 ,酶联免疫分析其活性 ,并应用荧光显微镜技术对乳鼠心肌细胞凋亡进行检测 ,荧光分光光度计测定细胞内钙离子浓度。结果 1 0 - 6mol/L ,1 0 - 5mol/L和 1 0 - 4mol/L川芎嗪可显著减少LPS刺激的巨噬细胞COX 2mRNA和蛋白的表达 (P <0 0 5 ,P <0 0 1 ) ,但不同浓度川芎嗪对COX 2活性无明显作用 ;1 0 - 5mol/L川芎嗪可显著降低心肌细胞内钙浓度 ,拮抗LPS诱导的乳鼠心肌细胞凋亡 (P <0 0 5 )。结论川芎嗪具有抑制LPS诱导巨噬细胞COX 2表达和乳鼠心肌细胞凋亡的药理学效应。

英文摘要:

Objective To observe the effect of tetramethylpyrazine (TMP) on lipopolysaccharides (LPS) induced macrophage cyclo oxidase 2 (COX 2) gene expression and activity in RAW264.7 mice, and to further investigate the effect and mechanism of TMP on LPS induced apoptosis of cardiac myocytes in suckling mice. Methods RT PCR and Western Blot (WB) were used to investigate the macrophage COX 2 gene expression, ELISA was used to measure its activity, fluorescence microscopy was used to determine the apoptosis of murine neonatal cardiac myocyte, and fluorescence spectrophotometry was used to detect the concentration of intracellular calcium ion (Ca 2+ ). Results TMP of 10 -6 mol/L could significantly reduce the COX 2 mRNA and protein expression (P<0.05), in 10 -5 mol/L and 10 -4 mol/L could significantly decrease the COX 2 expression (P<0.01) stimulated by LPS, but couldn’t influence the activity of COX 2 by different TMP concentration. TMP in 10 -5 mol/L could significantly lower the concentration of intracellular Ca 2+ in cardiac myocyte, and antagonize the LPS induced apoptosis of cardiac myocyte in suckling mice (P<0.05).Conclusion TMP has the pharmacological effect in inhibiting LPS induced macrophage COX 2 expression and apoptosis of cardiac myocyte in suckling mice.

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