川芎嗪与左旋精氨酸对急性心肌梗死大鼠的心肌保护作用

目的比较川芎嗪 (TMP)与川芎嗪和左旋精氨酸 (L arg)联合疗法对急性心肌梗死 (AMI)大鼠的心肌保护作用 ,并探讨其作用机制。方法采用垂体后叶素 ( pituitrin ,Pit)尾静脉注射复制大鼠AMI模型 ,设立正常对照组、模型组、TMP治疗组、TMP与L arg联合治疗组 ,用免疫组化法观察心肌梗死时P 选择素、E 选择素的表达情况 ,并测定血清肌酸激酶 (CK)、肌钙蛋白T(TnT)及心肌组织髓过氧化物酶 (MPO)的浓度。结果模型组与正常对照组比较 ,血清CK、TnT浓度、心肌组织MPO浓度有明显升高 (P <0 0 1 ) ,P 选择素、E 选择素的表达明显上调 (P <0 0 1 ) ;TMP治疗组、联合治疗组分别与模型组比较 ,上述各指标均下降 ,联合治疗组下降更为明显 (P值均 <0 0 5 )。结论TMP与L arg合用可通过多种环节抑制黏附分子的表达和降低白细胞的浸润 ,对治疗AMI有显著协同作用

英文摘要:

Objective To compare the protective effects of tetramethylpyrazine (TMP) alone and TMP and L arginine (TMP LA) combination on rats with acute myocardial infarction (AMI), and to explore its mechanism. Methods The rat model of AMI was established by via caudal vein injection of pituitrin. Experimental animal groups of normal, model, TMP treated and TMP LA (via abdominal cavity and caudal vein respectively) treated groups were established. Expression of P and E selectin, serum creatine phosphokinase (CK) and troponin T(TnT), and marrow peroxidase (MPO) concentration in myocardial tissue were determined by immunohistochemical stain. Results As compared with the normal group, serum CK and TnT level, and MPO concentration in myocardial tissue were significantly higher in the model group (P<0.01), with P and E selectin significantly up regulated (P<0.01). As compared with the model group, the above mentioned parameters in the TMP or TMP LA treated group was significantly lower (P<0.05). Conclusion Combined use of TMP and LA showed obvious synergism in treating AMI, by way of multi link inhibition on expression of adhesive factors and decrease of leucocyte infiltration.

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