栀子苷对局灶性脑缺血大鼠脑组织基因表达谱的影响

作者	单位
张小燕	北京师范大学生命科学院
张占军	北京中医药大学临床医学院
王忠	中国中医研究院西苑医院
应康	上海联合基因集团
左明雪	北京师范大学生命科学院

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中文摘要:

目的研究栀子苷对局灶性脑缺血大鼠脑组织基因表达谱的影响 ,探索栀子苷治疗脑缺血的药理作用机制。方法分别从局灶性脑缺血组、栀子苷治疗组SD大鼠的脑组织中抽提总RNA ,Cy3、Cy5荧光标记 ,反转录分别合成cDNA探针后 ,与含有 4 0 96条大鼠基因的BioStar基因表达谱芯片杂交 ,AxonGenepix 4 0 0 0B扫描仪扫描 ,GenePixPro 3 0软件分析表达信号。结果栀子苷治疗后差异表达的基因有70条 ,其中有 6 8条基因上调 ,2条基因下调。结论栀子苷对局灶性脑缺血大鼠脑组织基因表达具有调控作用 ,从分子水平阐释了中药清开灵注射液成分栀子苷的药理作用机制。

英文摘要:

Objective To explore the pharmacologic mechanism of gardenin in treating cerebral ischemia, by studying its effect on gene expression profile in brain of rats with focal cerebral ischemia (FCI). Methods Total RNAs were isolated from rats with FCI and those treated with gardenin. The mRNAs were reversely transcribed to cDNA with incorporation of fluorescent Cy5 or Cy3 dUTP to prepare hybridization probes. The PCR products of 4096 genes were spotted on the chip after a serial treatment. The mixed probes were hybridized to the cDNA microarray. Axon Genepix 4000B and GenePixPro 3 0 software were used to scan and analyze the fluorescent signals. Results In the group treated with gardenin, there were 70 genes had expression profiles different to that in the model group in the focal cerebral ischemic brain tissue, in which 68 were up regulated and 2 down regulated. Conclusion Gardenin has regulatory effect on the gene expression in rats with focal cerebral ischemia, which elucidates part of the pharmacologic mechanism of Qingkailing in molecular level.

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