仙贞片对糖尿病大鼠肾皮质终末期糖化终产物及其受体mRNA表达的影响

Effect of Xianzhen Tablet on Content of Advanced Glycosylation End Products (AGEs) and mRNA Expression of AGE-specific Cellular Receptor in Renal Cortex of Diabetic Rats

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DOI：

中文关键词: 仙贞片糖尿病糖基化终产物特异性受体信使核糖核酸

英文关键词:Xianzhen tablet diabetes mellitus end product of glycosylation specific receptor messenger ribonucleic acid

基金项目:

作者	单位
唐代屹	中国医学科学院中国协和医科大学病理生理学教研室北京100730
郭赛珊	中国医学科学院中国协和医科大学病理生理学教研室北京100730
孙仁宇	中国医学科学院中国协和医科大学病理生理学教研室北京100730

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中文摘要:

目的观察仙贞片对糖尿病大鼠肾皮质终末期糖化终产物 (advancedglycationendproducts,AGEs)含量及其糖化终产物特异性受体 (AGE specificcellularreceptor,RAGE)信使核糖核酸 (messengerri bonucleicacid ,mRNA)表达的影响 ,探讨其对糖尿病大鼠肾保护的作用机制。方法采用链脲佐菌素(streptozotocin ,STZ)复制糖尿病持续性高血糖肾损害大鼠模型 ,用荧光测定法和逆转录 -多聚酶链式反应(reversetranscriptionpolymerasechainreaction ,RT PCR)技术检测模型大鼠肾皮质AGEs含量及RAGEmRNA的表达 ,与氨基胍作对照。结果实验 12周模型大鼠肾皮质AGEs相对含量及RAGEmRNA表达明显高于正常对照组 (P <0 0 5 ) ,仙贞片及氨基胍治疗组肾皮质AGEs相对含量及RAGEmRNA表达明显低于模型组 (P <0 0 5 ) ,仙贞片与氨基胍组比较差异无显著性 (P >0 0 5 )。结论仙贞片能减轻糖尿病大鼠肾皮质内AGEs的积聚 ,下调RAGEmRNA的过度表达 ,与氨基胍相近似 ,具有抑制蛋白非酶糖基化的作用 ,可能是其肾保护作用的机制之一。

英文摘要:

Objective investigate the effect of Xianzhen tablet (XZT, a Chinese patent compound recipe) on advanced glycosylation end products (AGEs) and mRNA expression of AGE specific cellular receptor (RAGE) in renal cortex of diabetic rats in order to explore the mechanism of XZT in protecting kidney. Methods The diabetic rat model with persistent hyperglycemic renal damage was reproduced by streptozotocin. Fluorescent assay and RT PCR were used to determine the content of AGEs and expression of RAGE mRNA in renal cortex in model rats, which were treated with XZT and controlled by aminoguanidine (AG) administration. T5HZ]ResultsResults The relative content of AGEs and RAGE mRNA expression in renal cortex of model rats 12 weeks after modeling were significantly higher than those in the normal group ( P <0 05), while those in model rats treated by XZT or AG were markedly lower than those in non treated model rats ( P <0 05), the effect of the both groups showed insignificant difference ( P >0 05). Conclusion XZT could reduce the accumulation of AGEs in renal cortex of diabetic rats, down regulate the over expression of RAGE mRNA, with the effects similar to that of AG, the inhibition of XZT on protein non enzymatic glycosylation might be one of the mechanisms of its effect in protecting kidney.

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