复方肾华片对5/6肾切除大鼠肾组织巨噬细胞移行抑制因子的影响

作者	单位
李建军	解放军总医院肾科解放军肾病中心暨重点实验室北京 100853
陈香美	解放军总医院肾科解放军肾病中心暨重点实验室北京 100853
顾玥	解放军总医院肾科解放军肾病中心暨重点实验室北京 100853
魏日胞	解放军总医院肾科解放军肾病中心暨重点实验室北京 100853
杜靖	解放军总医院肾科解放军肾病中心暨重点实验室北京 100853
师锁柱	解放军总医院肾科解放军肾病中心暨重点实验室北京 100853
尹忠	解放军总医院肾科解放军肾病中心暨重点实验室北京 100853

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中文摘要:

目的观察复方肾华片(下称肾华片)对大鼠残余肾脏表达巨噬细胞移行抑制因子(macrophagemigration inhibition factor,MIF)的作用。方法采用肾华片治疗5／6肾切除大鼠12周,观察血压、尿蛋白、血生化、肾脏病理改变以及MIF表达的情况。结果肾华片能降低5／6肾切除大鼠血清肌酐水平(P<0.05)及24 h尿蛋白总量(P<0.01),减少肾小球及肾小管间质MIF表达和浸润的巨噬细胞数量,减轻肾小球硬化及肾间质纤维化程度。结论抑制MIF的高表达,可能是肾华片抑制大鼠残余肾脏慢性炎症,延缓硬化、纤维化过程,保护肾功能的机制之一。

英文摘要:

To observe the effect of compound Shenhua tablet (CST) on the residual kidney expressed macrophage migration inhibition factor (MIF) in rats. Methods CST was used to treat 5/6 nephrectomized rats for 12 weeks and the conditions of blood pressure, urinary protein, blood biochemical indices (creatinine, blood urea nitrogen), kidney pathologic change and MIF expression were observed. Results CST could significantly lower the serum levels of creatinine (P<0.05), and 24 hrs urinary protein (P<0.01), reduce the MIF expression and macrophage infiltration in renal glomerulus and tubular mesenchym, and lower the degree of renal glomerular sclerosis and interstitial fibrosis. Conclusion The inhibition on the highly expressed MIF may be an important mechanism of the drug in restraining chronic inflammation in residual kidney, delaying the sclerosis and fibrosis progression and protecting renal function.

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