缺血性中风中医辨证分型与ICAM-1和CD62P表达水平的相关性

作者	单位
祝美珍	广西中医学院基础医学院中医诊断学教研室南宁530001
李志刚	广西中医学院基础医学院中医诊断学教研室南宁530001

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中文摘要:

目的观察缺血性中风中医证型与血中性粒细胞表面细胞间黏附分子 (ICAM 1)和血小板膜上P选择素 (CD6 2P)变化的关系 ,探讨缺血性中风的发病机理。方法将 72例缺血性中风患者按中医辨证分为 3个证型组 :即痰瘀阻络型组、痰热腑实型组和气虚血瘀型组 ,每组 2 4例 ,另设一健康人对照组 (2 4名 )。用流式细胞仪测定各组患者的ICAM-1、CD6 2P的表达水平。结果缺血性中风患者ICAM 1、CD6 2P水平均显著高于健康人对照组 (P <0 .0 1) ,其中痰瘀阻络型组ICAM 1的表达水平显著高于气虚血瘀型组和痰热腑实型组 (P <0 .0 1) ;痰瘀阻络型组和气虚血瘀型组CD6 2P的表达水平显著高于痰热腑实型组 (P <0 .0 1)。结论缺血性中风不同证型的ICAM-1、CD6 2P表达水平存在一定的差异性 ,ICAM-1反映了体内痰阻或痰瘀互结的病理状态 ,而CD6-2P则反映了体内血瘀状态。提示痰瘀阻络可能是缺血性中风的病机关键

英文摘要:

study the correlationship between TCM Syndrome type with changes of neutrophil surface intercellular adhesion molecule-1 (ICAM-1) and platelet membrane P selectin (CD62P) in patients with ischemic stroke for exploring the pathogenesis of the disease. MethodsSeventy-two patients with ischemic stroke were dividcd into 3 typing groups according to TCM syndrome-differentiation, the Meridian-phlegm stagnancy group (MPS), the visceral phlegm-heat accumulation group (VPHA) and the qi-deficiency with blood stasis group (QDBS), 24 in each group. Besides, a control group consisted of 24 healthy subjects was set up. Blood levels of ICAM-1 and CDP62 expression were monitored by flow cytometry. ResultsBlood levels of ICAM-1 and CD62P expression in ischemic stroke patients were significantly higher than those in healthy subjects (P<0.01). Among the three type groups, ICAM-1 expression was significantly higher in MPS than that in the VPHA and the QDBS group (P<0.01), and CD62P expression in the MPS and the QDBS group was significantly higher than that in the VPHA (P<0.01). Conclusion Blood levels of ICAM-1 and CD62P expression in different typing of patients with ischemic stroke are different. ICAM-1 expression reflects the pathological state of phlegm retention or phlegm-stasis mutual bindings, CD62P expression reflected the blood stasis state in organism, these evidences suggest that MPS may be the key pathogenic factor of ischemic stroke.

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