黄芪注射液与白细胞介素2增强树突细胞抗肿瘤转移作用的比较研究

作者	单位
董竞成	复旦大学附属华山医院中西医结合肺和特应性疾病研究室上海200040
董晓辉	复旦大学附属华山医院中西医结合肺和特应性疾病研究室上海200040

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中文摘要:

目的观察比较黄芪注射液与白细胞介素 (IL)-2增强树突细胞 (DCs)的抗肿瘤转移作用。方法制备C5 7BL/ 6小鼠骨髓来源的DCs,用Lewis肺癌特异性多肽Mut1预激DCs,并联合黄芪注射液或IL-2治疗转移性肺癌小鼠 ,通过流式细胞仪分析其脾细胞内T淋巴细胞亚群比例的变化 ,用Elisa法检测荷瘤小鼠血清IL-2、IL-4含量。结果肿瘤抗原多肽致敏的DCs与黄芪注射液或IL-2联合治疗后 ,肺癌结节减少 ,小鼠脾脏中T淋巴细胞亚群 (CD4 +和CD8+)升高 ,血清IL-2 /IL-4比值也明显升高。在观察期间 ,经DCs联用IL-2和DCs联用黄芪注射液免疫小鼠成瘤率均低于单用DCs组。结论黄芪注射液和IL-2均能增强DCs的抗肿瘤转移作用 ,有效地促进荷瘤宿主的免疫应答 ,具有显著的体内抑制肺癌转移的效果。对正常动物免疫保护作用更加明显

英文摘要:

compare the effect of Astragulus injection (AGI) and interleukin-2 (IL-2) in enhancing anti-tumor metastasis action of dendrite cells (DCs) based vaccine. MethodsC57BL/6 mice’s myelogenic DCs were prepared and pre-sensitized by Mut1, a MHC class I-restricted tumor antigen polypeptide of Lewis lung cancer. Then the DCs were used to treat mice with metastatic lung cancer in combination with AGI or IL-2. Change of proportion of T-lymphocyte cell subsets in splenic cell was analyzed by flow cytometry, and the serum contents of IL-2 and IL-4 of the tumor bearing mice were detected by ELISA. ResultsAfter being treated with tumor antigen polypeptide sensitized DCs plus AGI or IL-2, the tubercle of lung cancer decreased, proportion of subsets CD4 +T and CD8 +T in mice’s splenic cell increased, and serum IL-2/IL-4 ratio also increased obviously. During the observed period, the tumor developing rate in the immune mice treated with DCs combined treatment, either with IL-2 or with AGI, was lower than that in mice treated with DCs alone. ConclusionBoth AGI and IL-2 can enhance the anti-tumor metastasis action of DCs, effectively promote the immune response of tumor bearing host, therefore have obviously inhibitory effect on lung cancer metastasis in vivo. Their immune protective function in normal animals is even more evident.

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