| 柴立民,吴志奎,张新华,蔡辉国.中药益髓生血颗粒对β-珠蛋白生成障碍性贫血患儿基因表达的影响[J].中国中西医结合杂志,2005,(7):591-594 |
| 中药益髓生血颗粒对β-珠蛋白生成障碍性贫血患儿基因表达的影响 |
| Effect of Yisui Shengxue Granule on Gene Expression in Children with β-Thalassemia |
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| DOI： |
| 中文关键词: 益髓生血颗粒 β-珠蛋白生成障碍性贫血 mRNA差异显示技术 铁蛋白 |
| 英文关键词:Yisui Shengxue granule β-thalassemia mRNA differential display ferrintin |
| 基金项目:国家自然科学基金资助项目(No.30171199);国家自然科学基金重大研究计划(No.90409003) |
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| 中文摘要: |
| 目的探讨益髓生血颗粒治疗β珠蛋白生成障碍性贫血的作用机制。方法观察20例患儿治疗前后血红蛋白(Hb)、红细胞(RBC)、网织红细胞计数(Ret)和胎儿血红蛋白(HbF)的改变;选取3例有效病例,通过mRNA差异显示技术(DDRTPCR)研究益髓生血颗粒对β珠蛋白生成障碍性贫血患儿骨髓有核细胞的基因表达差异。结果与治疗前比较治疗后患儿各项血液学参数均有提高(P<0.05或P<0.01);患儿骨髓有核细胞的铁蛋白基因表达明显降低。结论益髓生血颗粒可以改善患儿的临床症状,明显降低患儿铁蛋白基因的表达,有效缓解患儿体内铁的蓄积,这可能是益髓生血颗粒治疗本病的分子机制之一。 |
| 英文摘要: |
| ObjectiveTo investigate the mechanism of Yisui Shengxue granule (YSG) in children with β-thalassemia. MethodsThe changes of hemoglobin (Hb), red blood cells (RBC), reticulocyte (Ret) and fetal hemoglobin (HbF) of 20 children with β-thalassemia were measured before and after treatment. The effect of YSG on gene expression in myelo-karyocytes in 3 selected children treated effectively by YSG was determined by using DDRT-PCR. ResultsThe hematologic parameters were significantly enhanced and the ferritin gene expression declined after treatment respectively, as compared with those before treatment, the difference were significant (P<0,01 or P<0.05). ConclusionYSG could improve the clinical symptoms of children with β-thalassemia. One of its therapeutic mechanisms may be its action in decreasing ferritin gene expression so as to effectively relieve the accumulation of iron in body. |
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