清肺口服液对3I、7b型腺病毒感染人胚肺成纤维细胞转化生长因子-β1和血小板衍生生长因子-BB蛋白表达的影响

Effect of Qingfei Oral Liquid on Protein Expression of Transforming Growth Factor-β₁ and Platelet Derived Growth Factor-BB of Adenovirus Type 3Ⅰ,7b Induced Human Embryonic Lung Fibroblast Cells

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DOI：

中文关键词: 清肺口服液人胚肺成纤维细胞腺病毒转化生长因子-β1 血小板衍生生长因子-BB

英文关键词:Qingfei oral liquid human embryonic lung fibroblast cells adenovirus transforming growth factor-β₁ platelet derived growth factor-BB

基金项目:国家自然科学基金项目(No.30171171)

作者	单位
王文革	南京中医药大学儿科南京210029
陈四文	南京中医药大学儿科南京210029
汪受传	南京中医药大学儿科南京210029

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中文摘要:

目的探讨清肺口服液含药血清对腺病毒3Ⅰ、7b感染的人胚肺成纤维细胞之转化生长因子β1(TGFβ1)、血小板衍生生长因子BB(PDGFBB)蛋白表达的影响。方法细胞分为正常细胞组、病毒对照组、空白血清组、利巴韦林组和含药血清组,除正常细胞组外,其余4组均以3Ⅰ、7b型腺病毒分别攻击之,并分别加入相应药物至各组细胞中;采用酶联免疫吸附(ELISA)法检测各组细胞上清液中TGFβ1、PDGFBB的含量。结果病毒对照组较正常细胞组细胞中TGFβ1、PDGFBB含量均明显增多(P<0.01),含药血清组比病毒对照组的细胞TGFβ1、PDGFBB含量显著降低(P<0.01)。结论(1)腺病毒感染可使人胚肺成纤维细胞TGFβ1、PDGFBB蛋白表达增高;(2)清肺口服液可降低TGFβ1、PDGFBB细胞因子的蛋白表达,这可能是其抗病毒作用的机制之一。

英文摘要:

ObjectiveTo explore the effect of Qingfei oral liquid （QOL） contained serum on protein expression of transforming growth factor-β₁ （TGF-β₁） and platelet derived growth factor-BB （PDGF-BB） of adenovirus type 3I,7b induced human embryonic lung fibroblast cells. MethodsThe cells were divided into 5 groups, the normal cells group （NCG）, the virus control group （VCG）, the blank serum group （BSG）, the ribavirin group （RVG） and the QOL contained serum group （QSG）. All the cells except those in the NCG were challenged by adenovirus type 3I, 7b and treated with correspondent medicine. The contents of TGF-β₁ and PDGF-BB in the supernatant of cell culture were monitored by ELISA and compared among groups.ResultsContents of TGF-β₁ and PDGF-BB in VCG were significantly higher, while those in QSG were significantly lower than those in VCG （P<0.01）. ConclusionAdenovirus infection can increase the protein expression of TGF-β₁ and PDGF-BB of human embryonic lung fibroblast cells. QOL can decrease the protein expression of these cytokines, which maybe one of the mechanisms of its antiviral effect.

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