黄芪注射液对大鼠左室重构及凋亡基因Caspase-3的影响

目的观察黄芪注射液对大鼠心肌梗死后左室重构及凋亡基因caspase3的影响。方法制作心肌梗死大鼠模型,实验分为4组,Ⅰ组(假手术组)、Ⅱ组(假手术+黄芪组)、Ⅲ组(模型组)和Ⅳ组(模型+黄芪组)。Ⅱ和Ⅳ组大鼠术后每日腹腔注射黄芪注射液2ml,Ⅰ组和Ⅲ组大鼠给等体积生理盐水,连续用药4周后,观察左室结构变化,心肌羟脯氨酸含量及用免疫组化检测caspase3表达。结果与Ⅲ组比较,Ⅳ组心肌超微结构和左室重构指标改善,羟脯氨酸含量减少(P<0.05),caspase3阳性细胞明显减少,caspase3mRNA表达明显下调(P<0.05)。结论黄芪注射液能改善大鼠心肌梗死后左室重构,并下调凋亡基因caspase3表达,抑制凋亡。

英文摘要:

ObjectiveTo investigate the effect of Astragalus injection (AI) on left ventricular remodeling and the expression of apoptotic gene caspase-3 in rats after myocardial infarction (MI). MethodsThe MI model was established. The experimental animals were divided into 4 groups, Group Ⅰ (the sham-operated group), GroupⅡ (the sham-operated plus AI group), Group Ⅲ (the model group),Group Ⅳ (the model plus AI group). Animals in the Ⅱand Ⅳ group were intraperitoneally injected with 2ml AI once a day after operation, while animals in theⅠand Ⅲ group were treated with normal saline of equal volume. After treated for 4 weeks successively, the structural change of left ventricle and the level of oxyproline in myocardium were observed, and expression of caspase-3 was determined by immunohistochemical method. ResultsAs compared with Group Ⅲ, the ultrastructure of myocardium and indexes of left ventricular remodeling were improved, the myocardial content of oxyproline decreased (P<0.05), the caspase-3 positive cells reduced and caspase-3 mRNA expression significantly down-regulated (P<0.05). ConclusionAI can improve left ventricular remodeling, inhibit apoptosis by down-regulate the expression of apoptotic gene caspase-3 in rats after MI.

关闭